中文摘要：

目的观察脂肪干细胞（ADSCs）体外诱导分化成神经细胞的潜能，为恢复阴茎海绵体神经受损导致勃起功能障碍的研究建立基础。方法取SD雄性大鼠脂肪组织进行原代培养。流式细胞仪检测ADSCs，体外诱导成脂肪细胞和神经细胞并进行鉴定。结果ADSCs表面分子阳性率：CD44（＋）96．4％、CD45（-）1．7％、CD34（-）0．9％。成脂细胞油红O染色脂滴染成红色。神经细胞荧光染色胶原纤维酸性蛋白（GFAP）、β-微管蛋白（β—tubulin）Ⅲ蛋白表达阳性，方法1[表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、脑源性神经营养因子（BDNF）＋吲哚美辛、胰岛素、3-异丁基-1-甲基黄嘌呤（IBMX）]和方法2（EGF、bFGF＋吲哚美辛、胰岛素、IBMX）诱导率分别为（74．0±3．3）％、（65．3±2．1）％和（51．0±1．2）％、（41．0±1．1）％，且阳性细胞数差异有统计学意义（P〈0．01）。结论ADSCs在方法1作用下向神经细胞分化诱导率高，时间短；ADSCs可能成为治疗神经性勃起功能障碍的较理想干细胞。

英文摘要：

Objective To probe into the potential of neural differentiation of rat adipose-derived stem cells （ADSCs） in vitroto provide foundation to restore erectile dysfunction （ED） caused by cavernous nerves （CNs） injury. Methods Adipose tissue from SD rat abdomen and caputepididymidis was digested with collagenase type I, followed by filtering and centrifugation. The isolated adipose stromal cells were cultured in dishes. ADSCs markers were measured by flow cytometry. Cells at the third passage were used for in vitro differentiation. Adipogenic and neuronal differentiation was induced by incubation of ADSCs with different induction media. Oil red O staining was carried out to identify adipose cells, and immunoflu- orescence to detect protein of glial fibrillary acidic protein （GFAP） and beta tubulin RI. Results Flow cytometry demonstrated that the ADSCs expressed CD44, but CD45 and CD34 were weakly expressed, and their positive rate was 96.4% , 1.7% , and 0. 9% respectively. Adipose cells as evidenced by the lipid droplet were dyed red by oil red O dyeing. Neuron-like cells were evidenced by neuronal morphology and the presence of neuronal markers including GFAP and β-tubulin III. The induction rate of GFAP and β-tu- bulin III by method 1 [ epidermal growth factor （EGF）, basic fibroblast growth factor （bFGF）, brain-de- rived neurotrophic factor （BDNF） ＋ indomethaein, insulin, and 3-isobutyl-l-methylxanthine （IBMX） ] and method 2 （EGF, bFGF ＋ indomethaein, insulin, and IBMX） was （74. 0 ± 3.3 ） % and （65.3 ± 2. 1 ） % , and （51.0 ± 1.2） % and （41.0 ± 1.1 ） % respectively. The number of the positive cells exhibi- ted significant difference after the induction （ P 〈 0. 01 ）. Conclusion ADSCs can be easily obtained from a small amount fat tissue and developed in culture. By using the method 1, the induction rate is high and induction time is short in neural differentiation of ADSCs. ADSCs may be an alternative source of stem cell therapy for neuropathie ED.