中文摘要：

趋化性的活动性涉及许多病原的细菌的毒力。以便在细胞的过程和毒力理解 Vibrio harveyi 的趋化性的活动性的角色， mini-Tn10/Kan 有缺乏的趋化性的活动性的导致 transposon 的异种被构造，屏蔽，并且识别。顺序分析表明 465-bp 碎裂(情报银行就职数字 HM630274 ) 在变异的 TS-CM1 的 transposon 插入地点有的 flanking 有 V 的假想蛋白质基因的最高的身份(96.9%) 。有 V 的 pgk 基因的 harveyi ATCC BAA-1116 和秒高度身份(91.8%) 。parahaemolyticus RIMD 2210633。在另一异种， TS-CM2，胁腹 transposon 顺序(情报银行就职数字 HM630275 ) 的 356 bp 也与 V 的假想蛋白质基因显示出最高的身份(94.6%) 。有 V 的松驰的肌肉基因的 harveyi ATCC BAA-1116 和秒高度身份(92.4%) 。alginolyticus HY9901。象 themutants 的生长，活动性，粘附，和传染性那样的毒力相关的生物特征上的研究显示出 flagellin 基因或导致的精力新陈代谢基因的那混乱在病原的 V 的生长，活动性，粘附，和毒力的趋化性的活动性和变化的随后的损失。harveyi。因此， flagellin 基因和关键精力新陈代谢基因在 V 的趋化性的活动性起了一个重要作用。harveyi。

英文摘要：

Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-TnlO/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and iden- tified. Sequence analysis revealed that the 465-bp fragment （GenBank accession number HM630274） fank- ing the transposon insertion site in mutant TS-CM1 had the highest identity （96.9%） with a hypothetical protein gene of V. harveyiATCC BAA-1116 and the second-highest identity （91.8%） with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence （GenBank accession number HM630275） also showed the highest identity （94.6%） with a hypothetical pro- tein gene of V. harveyi ATCC BAA-1116 and the second-highest identity （92.4%） with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth, motility, adhe- sion, and infectivity of the mutants showed that disruption of either the flagellin gene or energy metabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and viru- lence of the pathogenic E harueyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotactic motility of V. harveyi.