中文摘要：

目的：观察丙泊酚麻醉对成年大鼠空间认知功能及海马CA1区GSK-3β水平的影响。方法：成年SD大鼠经尾静脉注射丙泊酚麻醉6 h,苏醒后24 h接受莫里斯水迷宫（Morris Water Maze,MWM）训练,观察全身麻醉对大鼠认知功能的影响;采用免疫荧光和Western blot技术,检测大鼠海马CA1区GSK-3β水平的变化。结果：与对照组相比,丙泊酚麻醉组大鼠在MWM任务的隐藏平台（P〈0.01）、反向平台（P〈0.01）和空间探索实验（P〈0.05）的空间学习和记忆能力显著下降;同时伴有CA1区磷酸化的GSK-3β（p-GSK-3β）表达水平的下降（P〈0.05）,GSK-3β水平不变（P〉0.05）。结论：丙泊酚麻醉可引起成年大鼠空间学习和记忆功能损害,其机制可能与降低海马CA1区的GSK-3β的磷酸化水平有关。

英文摘要：

Objective： To investigate whether propofol impairs the spatial learning and memory abilities and alters the level of GSK- 3β（ glycogen synthase kinase- 3beta,GSK- 3β） in the CA1 of hippocampus in adult rats.Methods： Rats received propofol anesthesia via intravenous injection for 6 h. Moriss Water Maze training was conducted 24 h after the end of anesthesia to observe the effect of propofol anesthesia on learning and memory function in SD adult rats,and the level of GSK- 3β in the CA1 in rats was detected by Western blot and immunofluorescence.Results： Propofol anesthesia impaired learning and memory associated with hidden platform and reversal platform training,as well as probe test in adult rats. Moreover,the level of p- GSK- 3β in the CA1 of rats decreased significantly after propofol treatment.Conclusion： Propofol anesthesia induces spatial learning and memory impairment in adult rats,which may be associated with the decrease of p- GSK- 3β in the CA1.