中文摘要：

从宁夏大武口高硫煤矸石山的酸性废水中筛选分离得到一株自养硫细菌，形态生理生化试验确定菌株T．f为氧化亚铁硫杆菌。应用该菌进行煤矸石的脱硫实验，溶液中的铁离子浓度呈现波状动态变化。在煤矸石粒径小于2mm，初始pH为1．5时，14d时硫酸根浓度增加7．5g／L，脱硫量为2．72g／L，脱硫率可达77.6％。该项目为改良煤矿矸石山废弃基质奠定了良好的条件，也为煤矿废弃物矸石山的生物脱硫以及生态恢复提供技术支持。

英文摘要：

Chemolithoautotrophic ferrous oxidizing bacteria is isolated by the enrichment and purification from the acid coal waste slurry in the Dawukou coal mining areas, T.f is determined as a kind of Thiobacillus ferrooxidans basicially through patterns physiological biochemical experiments, and applied in desulfurization experiments of coal wastes. The results show the iron ions concentration of solution changes undulatedly. When the particle size of coal wastes smaller than 2mm, the initial pH of 1.5, the SO4^2- concentration increase 7.5 g/L, the desulfurization volume is 2.72g/L and desulfurization rate of 77.6 %. It is not only the base for improving abandoned substance, but also technical support for biodesulfurization and their ecological restoration of coal mining wastes.