中文摘要：

目的探讨1-溴丙烷（1-BP）亚急性吸入染毒对雄性大鼠血浆和脑组织中神经元特异性烯醇化酶（NSE）和髓鞘碱性蛋白（MBP）的影响。方法无特定病原体级成年雄性Wistar大鼠随机分为对照组和低、中、高剂量组,每组12只,分别予质量浓度为0、1 250、2 500、5 000 mg/m3的1-BP连续动式吸入染毒,每天染毒6 h,每周5 d,连续4周。染毒结束后,各组随机取9只大鼠,经腹主动脉采血,分离血浆,采用免疫酶联吸附法检测其NSE和MBP水平,分离全脑、大脑、小脑和脑干检测脏器系数;各组另3只大鼠行脑病理组织学检查,并采用免疫组织化学法检测脑组织中NSE和MBP的蛋白表达。结果高剂量组大鼠全脑、大脑、小脑及脑干的脏器系数分别高于对照组[（0.754±0.056）%vs（0.663±0.035）%,（0.382±0.037）%vs（0.339±0.021）%,（0.115±0.008）%vs（0.098±0.006）%,（0.213±0.018）%vs（0.183±0.014）%,P〈0.01]。低、中和高剂量组大鼠血浆中NSE水平分别低于对照组[（7.92±0.53）vs（24.73±11.44）mg/L,（9.12±2.17）vs（24.73±11.44）mg/L,（11.10±2.84）vs（24.73±11.44）mg/L,P〈0.01];4组大鼠血浆中MBP水平比较,差异无统计学意义[（2.52±0.70）vs（2.50±0.72）vs（2.47±0.66）vs（2.44±0.81）mg/L,P〉0.05]。脑组织病理学检查结果显示：仅在高剂量组大鼠大脑海马区观察到少量坏死的神经元。低、中剂量组和对照组大鼠脑组织中NSE、MBP的蛋白表达量无明显差异,仅在高剂量组大鼠大脑海马区观察到NSE和MBP蛋白表达量下调的细胞。结论 1-BP所致的神经毒性对中枢神经功能影响比结构影响更为明显;血浆中NSE可能是1-BP暴露的效应标志物之一。

英文摘要：

Objective To determine the effects of 1-bromopropane（ 1-BP） subacute inhalation on the expression of neuron specific enolase（ NSE） and myelin basic protein（ MBP） in plasma and brain tissue in male rats. Methods Specific pathogen free adult male Wistar rats were randomly divided into 4 groups with 12 rats in each group： the control group,the low-,medium- and high-dose groups with 1-BP exposure levels at 0,1 250,2 500 and 5 000 mg / m3,respectively. The rats were given continuous dynamic inhalation of 1-BP for 6 hours per day,5 days per week,for continuous 4 weeks. The rats were sacrificed at the end of exposure,9 rats from each group were randomly chosen and the blood from abdominal aorta was collected and the plasma was isolated. The plasma levels of NSE and MBP were measured by enzyme-linked immunosorbent assay. The whole brain,pallium,cerebellum and brainstem were isolated for detection of organ coefficient.The rest of 3 rats in each group were processed for histopathologic examination and the expressions of NSE and MBP were evaluated by immunohistochemistry. Results The organ coefficients of whole brain,pallium,cerebellum and brainstem in the high-dose group were higher than those in the control group [（ 0. 754 ± 0. 056） % vs（ 0. 663 ± 0. 035） %,（ 0. 382 ±0. 037） % vs（ 0. 339 ± 0. 021） %,（ 0. 115 ± 0. 008） % vs（ 0. 098 ± 0. 006） % and（ 0. 213 ± 0. 018） % vs（ 0. 183 ±0. 014） %,respectively,P 0. 01]. The plasma NSE levels in the 3 exposure groups were lower than those of control group [（ 7. 92 ± 0. 53） vs（ 24. 73 ± 11. 44）,（ 9. 12 ± 2. 17） vs（ 24. 73 ± 11. 44） and（ 11. 10 ± 2. 84） vs（ 24. 73 ±11. 44） mg / L,respectively,P 0. 01]. The plasma MBP levels in all groups showed no statistical difference [（ 2. 52 ±0. 70） vs（ 2. 50 ± 0. 72） vs（ 2. 47 ± 0. 66） vs（ 2. 44 ± 0. 81） mg / L,P 0. 05]. Histopathological examination showed that a few necrotic nerve cells were observed in hippocampus of rats in high-dose group.