中文摘要：

以海藻酸钠（NaAlg）为印迹材料,以牛血清白蛋白（ BSA）为模板分子,以聚丙烯（PP）无纺布为支撑体,制备了 PP无纺布支撑的BSA分子印迹海藻酸钙水凝肢膜（ PP-s-CaAlg MIP）. 同时不加蛋白质制备了非印迹膜,记为 PP-s-CaAlg NIP.通过扫描电镜（SEM）、红外光谱（FT-IR）对 PP-s-CaAlg凝肢膜进行表征,测定了其在生理盐水中的溶胀性能. 讨论了不同NaAlg浓度制备的PP-s-CaAlg水凝肢膜在不同缓冲液中对BSA的释放. 研究了不同缓冲液下不同NaAlg浓度制备的PP-s-CaAlg MIP膜 和 NIP膜对 BSA的吸附性能. 结果表明： PP-s-CaAlgMIP膜对BSA的吸附量明显优于PP-s-CaAlg NIP膜;与对比蛋白质相比, PP-s-CaAlg MIP对模板蛋白质的吸附量更多,表明其具有一定的吸附选择性;PP-s-CaAlg MIP膜制备简单,成本低,生物相容性好,可用于细胞培养和组织工程领域.

英文摘要：

Bovine serum albumin （BSA） imprinted calcium alginate hydrogel film supported by polypropylene （PP） non- woven fabrics （PP-s-CaAlg MIP） was prepared using sodium alginate as the molecularly imprinted materials,BSA as the template molecule, and PP non-woven fabrics as the matrix. At the same time the non-molecularly imprinted film was also prepared without BSA and named as PP-s-CaAlg NIP. The PP-s-CaAlg MIP was char-acterized by SEM and FT-IR, and its swelling performance was measured in physiological saline. The release properties of BSA on the PP-s-CaAlg hydrogel films prepared with different NaAlg concentrations were investi-gated in different buffers. The adsorption properties of the PP-s-CaAlg MIP and NIP films prepared with different NaAlg concentrations under different buffers were analyzed. The experimental results showed that the PP -s - CaAlg MIP film adsorbed more BSA than PP-s-CaAlg NIP film. Compared with contrast protein, PP-s-CaAlg MIP adsorbed more template protein, indicating that the PP-s-CaAlg MIP exhibited the performance of adsorp-tion selectivity. PP-s-CaAlg MIP exhibited good biocompatibility and could be prepared by a fast, low -cost method, which would find its potential applications in the fields of cell culture and tissue engineering.