中文摘要：

<正>Objective:San’ao Decoction(三拗汤,SAD),as a representative Chinese medicine(CM)formula, was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide (LPS)enhanced asthma model.Methods:The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin(OVA),challenged by OVA and LPS.After Balb/C mice’s administration of a dose(0.0024 g/kg)of dexamethasone acetate,and three doses(2.2 g/kg,4.4 g/kg and 8.8 g/kg)of SAD,airway inflammation and responsiveness were observed.The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF)cells and lung histopathology.Also,differential expressions of interferon-r(IFN-γ),interleukin-4(IL-4), and IL-5 in the supernatants of BALF were examined.The changes in airway responsiveness indicated by lung resistance(R_L)and stimulated by acetylcholine(Ach)were determined.Results:Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma,while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness,and to some extent,reduced airway inflammation. Meanwhile,the small-dose,medium-dose,and high-dose SAD promoted Th1-type cytokines(IFN-γ)and reduced Th2-type cytokines(IL-4,IL-5)to different extents,which led to a Th1/Th2 balance.Conclusion:SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model,but its definite influence on airway inflammation is not remarkable.

英文摘要：

Objective：San＇ao Decoction（三拗汤,SAD）,as a representative Chinese medicine（CM）formula, was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide （LPS）enhanced asthma model.Methods：The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin（OVA）,challenged by OVA and LPS.After Balb/C mice＇s administration of a dose（0.0024 g/kg）of dexamethasone acetate,and three doses（2.2 g/kg,4.4 g/kg and 8.8 g/kg）of SAD,airway inflammation and responsiveness were observed.The airway inflammation was detected by counting bronchoalveolar lavage fluid （BALF）cells and lung histopathology.Also,differential expressions of interferon-r（IFN-γ）,interleukin-4（IL-4）, and IL-5 in the supernatants of BALF were examined.The changes in airway responsiveness indicated by lung resistance（R_L）and stimulated by acetylcholine（Ach）were determined.Results：Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma,while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness,and to some extent,reduced airway inflammation. Meanwhile,the small-dose,medium-dose,and high-dose SAD promoted Th1-type cytokines（IFN-γ）and reduced Th2-type cytokines（IL-4,IL-5）to different extents,which led to a Th1/Th2 balance.Conclusion：SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model,but its definite influence on airway inflammation is not remarkable.