中文摘要：

目的研究新的非甾体类抗炎药利克飞龙（Licofelone）对大鼠肾小球系膜细胞IL-18诱导的趋化因子Fractalkine表达的影响。方法体外培养大鼠肾小球系膜细胞。设立IL-18刺激组（加入10μg/LIL-18刺激肾小球系膜细胞24h）、Licofelone干预组（先用10、50、100μmol/L的Licofelone处理肾小球系膜细胞30min,再加入10μg/LIL-18刺激肾小球系膜细胞24h）、正常对照组（无IL-18刺激和Licofelone干预,加等量的9g/L盐水作为对照）。反转录-多聚酶链反应（RT-PCR）测定各组FractalkinemRNA表达量。ELISA法测定各组细胞培养上清液中Fractalkine蛋白量。结果正常对照组FractalkinemRNA的相对表达量为179.0±21.0,IL-18刺激24h后,肾小球系膜细胞中趋化因子FractalkinemRNA表达上调为1220.1±185.7,与正常对照组比较有显著性差异（t=9.646P〈0.01）。10、50、100μmol/L的Licofelone呈剂量依赖性拮抗IL-18诱导的FractalkinemRNA表达上调,FractalkinemRNA的相对表达量依次为899.7±85.3、369.6±36.7、236.6±35.6,与IL-18刺激组比较有显著性差异（t=2.798、7.780、9.006Pa〈0.05）。IL-18刺激24h,肾小球系膜细胞中趋化因子Fractalkine蛋白表达量为（2097.4±293.8）ng/L,显著高于正常对照组（380.4±31.1）ng/L（t=10.068P〈0.01）。10、50、100μmol/L的Licofelone呈剂量依赖性拮抗IL-18诱导的Fractalkine蛋白表达上调,细胞培养上清中Fractalkine蛋白量依次为（1257.9±245.6）、（908.2±155.3）、（650.7±126.6）ng/L,与IL-18刺激组比较有显著性差异（t=3.798、6.199、7.834Pa〈0.05）。结论Licofelone拮抗肾小球系膜细胞中IL-18诱导的趋化因子Fractalkine基因表达。Licofe-lone在治疗免疫性肾损伤方面有一定的应用前景。

英文摘要：

Objective To study the effect of Licofelone,a novel non-steroid anti-inflammatory drug,on the expression of Fractalkine induced by interleukin-18（IL-18） in mesangial cells.Methods Rat mesangial cells were cultured and divided into IL-18 stimulated group,Licofelone-treated group and normal control group.The cells in IL-18 stimulated group were stimulated by 10 μg/L IL-18 for 24 h.In Licofelone-treated group,ahead of exposure of IL-18 for 24 h,cells were treated with Licofelone in the doses of 10,50 and 100 μmol/L for 30 min.Additionally,the mesangial cells without treatment of IL-18 and Licofelone were used as normal control group.Reverse transcription-polymerase chain reaction（RT-PCR） was used to measure the level of Fractalkine mRNA.The expressions of Fractalkine protein in every group were detected with enzyme linked immunosorbent assay （ELISA）.Results In normal control group,the expression level of Fractalkine mRNA was 179.0±21.0.After exposure of IL-18 for 24 h,the level of Fractalkine mRNA was 1 220.1±185.7,which was higher than that in normal control group （t=9.646 P〈0.01）.Licofelone inhibited IL-18 induced increase of Fractalkine mRNA expression in a dose-depended way in mesangial cells.In Licofelone-treated group,the levels of Fractalkine mRNA in cells exposed to 10,50 and 100 μmol/L Licofelone were 899.7±85.3,369.6±36.7 and 236.6±35.6,respectively,which were all higher than that of IL-18 stimulated group （ t=2.798,7.780,9.006 Pa〈0.05）.After stimulation of IL-18 for 24 h,the Fractalkine protein level was （2 097.4±293.8） ng/L in mesangial cells,which was significantly higher than that in normal control group[（380.4±31.1） ng/L]（t=10.068 P〈0.01）.Licofelone inhibited IL-18 induced increase of Fractalkine protein expression in a dose-depended way in mesangial cells.In Licofelone treated group,the levels of Fractalkine protein in cells exposed to 10,50 and 100 μmol/L Licofelone were（1 257.9±245.6）,（908.2±155.3） and （650.7±126.6） ng/L,respectively,w