中文摘要：

目的：探讨心肌肥大细胞聚集和脱颗粒,在小型猪冠状动脉微血管栓塞后心肌胶原纤维沉积中的作用。方法：32头小型猪分成4组。分别为冠状动脉微血管栓塞组（n=8）,假手术组（n=8）,肥大细胞膜稳定剂曲尼司特预处理并冠脉微血管栓塞组（n=8）,以及曲尼司特预处理但无冠脉微血管栓塞组（n=8）。冠脉微血管栓塞30 d后,用组织化学方法和透射电镜,研究肥大细胞总量以及脱颗粒肥大细胞的数量和胶原纤维的沉积。结果：微血管栓塞30 d后,肥大细胞总量和脱颗粒的肥大细胞数量以及胶原容积分数,同对照组相比均有显著性差异（P〈0.01）。而肥大细胞膜稳定剂曲尼司特能,有效减少微血管栓塞组中肥大细胞总量和脱颗粒的数量以及胶原容积分数。胶原容积分数分别同总的肥大细胞数量（r=0.91,P〈0.001）和脱颗粒的肥大细胞数量（r=0.92,P〈0.001）呈正比。结论：肥大细胞的聚集和脱颗粒促使心肌胶原纤维的沉积。

英文摘要：

Objective： To investigate potential pathophysiological role of cardiac mast cells accumulation and degranulation on the collagen deposition after coronary microembolization（CME）.Methods： CME was induced in miniswine by selective infusion of 15×104 microspheres（diameter,45 μm） into the left anterior descending artery groups（CME group,n=8）.Some CME-induced animals were pretreated with the MC stabilizer tranilast（50 mg/kg,twice daily）,beginning 2 weeks before CME and thereafter throughout the experimental period（CME ＋ tranilast group,n=8）,while some animals received tranilast without CME（tranilast group,n=8）.Eight sham-operated animals without CME served as controls.After 30 days,the total number of MC and degraunlating MCs and collagen deposition was assessed by histological and electronic microscopy studies.Results： The numbers of total and degranulating MCs and collagen volume fraction（CVF） at day 30 in CME group were significantly higher than those in controls（P0.01）.Treatment with tranilast significantly reduced the numbers of total and degranulating MCs and CVF at day 30（all P 0.01）.There was a significant positive correlation of the CVF with the number of total MCs（r=0.91,P0.001） and degranulating MCs（r=0.92,P0.001） over the CME myocardium.Conclusion： MCs accumulation and degranulating contribute to myocardial fibrosis collagen deposition.