中文摘要：

研究了白腐真菌黄孢原毛平革菌在不同营养条件下对五氯酚的降解．采用土豆葡萄糖液体培养基，接种后第2天投加PCP，在胞外木质素降解酶活性极低的真菌反应体系中，五氯酚能够有效降解，4d内1Omg·1^-1五氯酚降解率达到98％；采用高氮培养基，接种后第6天投加五氯酚，在胞外木质素酶含量极低的真菌反应体系和不含胞外酶的菌丝体反应体系中，五氯酚均能有效降解，1d内降解率分别达到56％和22％；采用低氮培养基，接种后第6天投加五氯酚，在胞外木质素酶含量较高的真菌反应体系、胞外酶液反应体系和不含胞外酶的菌丝体反应体系中，五氯酚均能有效降解，1d内降解率分别达到86％，17％和46％．结果表明。黄孢原毛平革菌在非木质素降解条件下，依赖胞内酶能有效地降解PCP；在木质素降解条件下依赖胞外木质素降解酶和胞内酶降解五氯酚，降解效率高于非木质素降解条件。

英文摘要：

The degradation of pentaehlorophenol （PCP）by the white-rot fungus Phanerochaete chrysosporium was studied under different nutrient condition. Inoculated in potato dextrose broth culture media at 2nd day, Phanerochaete chrysosporium can degrade 10mg·l^-1 PCP at rate of 98% in 4 days, when the extra-cellular liguin-degrading enzyme activity is less than 8U·l^-1. On the other hand, when it was inoculated in the high nitrogen media, the degradation rates of PCP by the whole fungus culture and mycelium can reach 56% and 22% respectively in one day, while in low nitrogen media, the degradation rate of PCP by the whole fungus culture, mycelium as well as extra-cellular enzyme were 86%, 46% and 17% respectively. The degradation of PCP by both of lignin-degrading enzyme and intracellular enzymes under liguinolytic condition is more efficient than that under nonligninolytic condition with only intracellular enzymes existent.