中文摘要：

采用pib基因启动子3＇端缺失,将最下游的TATAbox、CAATbox和暗诱导元件YTCANTYY一起敲除来研究这两类顺式作用元件在该启动子中的功能和相互关系。结果显示：TATAbox和CAATbox以及3＇端序列缺失都不能使pib启动子丧失其启动活性,依旧保持其根部高效表达的特异性。含2、4和6个黑暗诱导元件YTCANTYY的pib启动子均具有暗诱导启动活性,24h暗处理后的3个构建转基因水稻（Oryza sativa ssp.japonica）根部的GUS活性都显著增加,茎、叶虽有增加但不明显,这表明pib基因启动子的暗诱导启动活性主要由YTCANTYY暗诱导元件所决定的,它不仅能够独立响应暗诱导还能补偿TATA、CAAT所缺失的功能。

英文摘要：

To investigate promotion functions and interactions between TATA/CAAT boxes and darkness inducing motif YTCANTYY in pib promoter,a set of recombined binary plasmids of 3＇end deleted pib promoter were constructed.Experiment results from analysis of their transgenic rice （Oryza sativa ssp.japonica） plants showed that the deletion of pib promoter fragments of TATA and CAAT boxes in 3＇ end remained not only their promotion activity but also their high expression in root system.The 3＇ deleted pib promoter harboring 2 or 4 YYTCANTYY and intact pib promoter harboring 6 YTCANTYY all possessed darkness inducing activity.After 24 h darkness treatment,GUS activities in the transgenic rice plant roots of above three recombined binary plasmids enhanced clearly and the increases of GUS activities in the stem and leaf were not obvious.These were said that the darkness inducing activity in pib promoter is mostly decided by the motif of YTCANTYY and this motif also can compensate for the missing fuction of TATA/CAAT.