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二氧化硅诱导的肺纤维化大鼠肺组织及血浆中热休克蛋白70的变化
  • ISSN号:1001-9391
  • 期刊名称:《中华劳动卫生职业病杂志》
  • 时间:0
  • 分类:R735.7[医药卫生—肿瘤;医药卫生—临床医学]
  • 作者机构:[1]河北联合大学,唐山063000, [2]军事医学科学院卫生学与环境医学研究所
  • 相关基金:国家自然科学基金重点目(30430590);河北省科技支撑项目(092761940)
中文摘要:

目的探讨热休克蛋白70(heat shock protein70,Hsp70)在二氧化硅诱导的大鼠肺纤维化过程中的变化。方法48只Wistar大鼠随机分成对照组和染尘组,每组24只,分别观察7、14、21、28d。染尘组经气管内灌注二氧化硅(50mg/ml),对照组予等剂量的生理盐水。HE染色观察病理学变化,蛋白免疫印迹(Western bolt)法和免疫组织化学法检测肺组织中Hsp70的表达与定位,双抗夹心ELISA检测血浆中Hsp70含量的变化。结果染尘组染尘后7d肺组织中Hsp70含量升高,14d达到高峰,随后降低,但仍高于正常对照。染尘组14、21、28d时血浆中Hsp70含量明显高于生理盐水对照组,差异有统计学意义(P〈0.05)。染尘组血浆中Hsp70含量21d达到峰值[(0.216±0.027)μg/ml]。结论在染尘组大鼠肺组织和血浆中Hsp70含量均明显升高,并随肺纤维化进程出现先升高后降低的趋势,提示肺组织中Hsp70在二氧化硅诱导的肺纤维化过程中有重要的生物学作用。

英文摘要:

Objective To investigate the dynamic expression of Heat shock protein 70 (Hsp70) in the lungs and plasma of rats with pulmonary fibrosis induced by silicon dioxide (SiO2). Methods Forty-eight Wistar rats were randomly divided into the control group exposed to normal solution and group exposed to SiO2 (50 mg/ml) with intratracheal injection. Each group was divided into four subgroups. The animals of SiO2 group and control group were sacrificed and lungs were collected on the 7th, 14th and 28th days after exposure, respectively. The left lung tissues were examined with the histopathologic HE staining. The expression and localization of Hsp70 protein in the lung tissues were examined with western blot assay and immunohistochemistry, respec- tively. The expression levels of Hsp70 protein in the plasma were measured by ELISA. Results The expression of Hsp70 in lung tissues of SiO2 group increased on the 7th day and reached the peak value on the 14th day then decreased, but still was significantly higher than that of the control group, the expression of Hsp70 in plasma of SiO2 group still was significantly higher than that of the control group (P〈0.05). The maximum expression level of Hsp70 in plasma of SiO2 group on the 21st day after exposure was 0.216 ± 0.027 μg/ml. Conclusion The expression levels of HspTO protein in the lung tissues and plasma of the group exposed to SiO2 significantly increased, which were associated with the process of pulmonary fibrosis. It was suggested that Hsp70 protein may play an important biological role in the pulmonary fibrosis induced by SiO2.

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期刊信息
  • 《中华劳动卫生职业病杂志》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中华医学会
  • 主编:
  • 地址:天津河东区华越道6号
  • 邮编:300011
  • 邮箱:cjoh1983@163.com
  • 电话:022-24333581
  • 国际标准刊号:ISSN:1001-9391
  • 国内统一刊号:ISSN:12-1094/R
  • 邮发代号:6-50
  • 获奖情况:
  • 2000年中华医学会优秀期刊银奖,2000年天津市优秀期刊奖,2001中华预防医学会优秀期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,荷兰文摘与引文数据库,美国生物医学检索系统,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2000版)
  • 被引量:16727