中文摘要：

目的研究BTB／POZ蛋白家族成员Kaiso在肺癌组织中的表达情况探讨其与临床病理参数、肺癌患者预后的相关性。方法应用免疫组织化学法检~IJKaiso蛋白在100例原发性非小细胞肺癌组织（其中68例备有完整的预后资料）和30例相应癌旁肺组织中的表达情况，应用Western blot的方法比较30例癌旁肺组织与配对肺癌组织中Kaiso相对表达量的差异。结果免疫组织化学染色表明Kaiso在正常支气管上皮细胞呈极微弱的浆表达，Westernblot检测结果表明肺癌组织中Kaiso蛋白的表达量显著高于正常肺组织的表达水平（n=30，P=0．000）。肺癌组织中浆阳性率为63．00％（63／100），Kaiso蛋白浆表达与高TNM分期（P=0．006）和淋巴结转移（P=0．004）成正相关。Kaiso蛋白浆表达与肺癌的不良预后有密切关系，阳性表达组术后5年生存率（22．22％）显著低于阴性组（64．00％，P=0．002，LogRank法）。Kaiso在细胞核中的阳性表达率仅为5．00％（5／100），但与临床病理因素无关。结论Kaiso蛋白浆表达增强与肺癌患者的恶性表型及不良预后正相关。

英文摘要：

Objective To study the expression of Kaiso in human non-small cell lung cancer（NSCLC） to explore its relationship with the clinicopathological significance and prognosis of NSCLC. Methods Immunohistochemical studies were performed on 30 cases of normal lung tissues and 100 cases of NSCLC, including 68 cases with complete follow-up records. The Kaiso protein expressions were detected by western blot in 30 fresh lung cancer tissues and their corresponding normal tissue. Results Kaiso was rarely expressed in the cytoplasm of normal bronchial epithelial cells. The results of Western blot showed that the expression of Kaiso protein in NSCLC was higher than that in normal lung tissue （n=30, p=0.000）. The positive ratio of Kaiso expression in cytoplasm of NSCLC was 63.00% （63/100）, and Kaiso expression was positively correlated with the TNM stage （P=O.006） and lymph node metastasis （P=0.O04）. The five years survival ratio of patients with Kaiso positive expression （22.22%） was lower than that of patients with Kaiso negative expression （64.00%, P =0.002, Log Rank method）. The positive ratio of Kaiso expression in nuclear was 5.00% （5/100）, and was not associated with the clinicopathological factors. Conclusions Cytoplasmic overexpression of Kaiso protein is correlated with malignant phenotype and poor prognosis of non-small-cell-lung cancer.