中文摘要：

建立了电化学检测表面固定捕获的野生型p53蛋白质的方法．首先在金电极表面形成巯基化的单链DNA探针／己硫醇（HT）混合自组装膜，随后巯基化的单链DNA探针与溶液中序列匹配的靶点DNA杂交，所形成的一致性双链DNA捕获溶液中的野生型p53蛋白质．p53分子表面的半胱氨酸残基采用巯基特异性试剂N-（2．乙基-二茂铁）马来酰亚胺（Fc—Mi）进行衍生．通过检测二茂铁的电化学信号来指示p53与一致性双链DNA之间的特异性相互作用．p53蛋白质与双链DNA的键合程度取决于双链DNA的序列．该方法可检测的p53最低浓度为1．33nmol·L^-1．

英文摘要：

A voltammetric method for the determination of surface-confined wild-type p53 protein has been developed. Consensus double-stranded oligonucleotide （ds-DNA） was first formed onto gold electrodes via hybridization of thiolated single-stranded DNA （ss-DNA）/hexanethiol （HT） mixed self-assembled monolayer with the complementary target DNA. The resultant consensus ds-DNA was subsequently used to capture wild-type p53 in solution. The cysteine residues on the exterior of the p53 molecules were derivatized with a thiol-specific reagent, N-（2-ferrocenylethyl）maleimide （Fc-Mi）. Well-defined voltammetric peaks that report on the interaction of p53 with the consensus ds-DNA were obtained. The level of p53 binding was found to be dependent on the sequence of the double-stranded （ds-DNA）. The present method can measure p53 concentration as low as 1.33 nmol·L^-1.