中文摘要：

以茄子单性结实品系D-10花后7d的单性结实果实为试材,并以在茄子单性结实抑制差减文库中差异表达的EST片段Z569为基础,利用RACE技术,获得一个934bp的cDNA全长序列,为甲硫氨酸亚砜还原酶A基因,命名为SmMsrA。基因编码区共600bp,编码199个氨基酸。氨基酸序列分析显示该基因编码的蛋白具有甲硫氨酸亚砜还原酶基因家族结构域和保守基本序列GCFWG,与杨树甲硫氨酸亚砜还原酶A（MsrA）蛋白三级结构相似性较高,为67%。蛋白多序列比对和进化树分析表明,SmMsrA与番茄MsrA蛋白的同源性最高（92%）,进化距离最近。采用荧光定量PCR对单性结实品系D-10和非单性结实品系03-2中不同结实性果实发育过程中SmMsrA的表达量进行测定,结果表明：在不同结实性的子房和果实发育过程中SmMsrA基因都有表达,单性结实品系在低温条件下开花当天子房中的SmMsrA的表达量最高。

英文摘要：

Using parthenocarpic fruit of eggplant（Solanum melongena）harvested seven days after anthesis from line D-10 as material,based on the EST Z569 from eggplant parthenocarpic suppression subtractive hybridization library,a full cDNA（934 bp）was cloned by rapid amplification of cDNA ends（RACE）.The cloned gene was Methionine sulfoxide reductase A（MsrA）gene（SmMsrA）,containing an open reading frame（600 bp）and encoding a protein of 199 amino acids.The predicted protein was a member of the peptide methionine sulfoxide reductase（PMSR）superfamily,and contained a conserved sequence GCFWG.The tertiary structure of SmMsrA was similar to the poplar MsrA model（2j89）with a similarity of 67%.Multiple sequence alignments and phylogenetic tree analyses showed that SmMsrA had the highest similarity（92%）with the tomato MsrA（accession number P54153）.The expression of SmMsrA was determined by real-time quantitative RT-PCR during the fruit development of line D-10（parthenocarpic fruits and seeded fruits）and line 03-2（unparthenocarpic fruits and seeded fruits）.The result showed that the SmMsrA gene was expressed throughout eggplant fruit development in different cultivars,and in parthenocarpic ovaries the highest expression was at anthesis day and under suboptimal temperatures.