中文摘要：

目的：研究TRPC6在大鼠触液核（cerebrospinal fluid-contacting nucleus，CSF—CN）中的分布及其在吗啡依赖与戒断条件下的表达变化。方法：雄性SD大鼠随机分为4组：正常大鼠组、生理盐水组、吗啡依赖组和吗啡戒断组。每组大鼠进行戒断总评分（total withdrawal scores，TWS）；采用侧脑室注射CB．HRP，并结合CB．HRPP／TRPC6免疫荧光双标技术，观察大鼠触液核内TRPC6的表达情况。结果：上述4组大鼠的戒断总评分分别为2．0±0．6、2．1±0．7、2．6±0．7和41．0±4．6，其中吗啡戒断组与其他三组相比，差异具有显著性（P〈0．01）；在侧脑室注射CB—HRP后，上述4组大鼠触液核内可观察到大量的CB—HRP标记神经元；同时在触液核内也可观察到部分神经元表达TRPC6免疫阳性。经计数，CB—HRP／TRPC6双标神经元的数量分别为81．78±4．93、79．44±7．09、254．61±15．36和260．00±12．04，其中吗啡依赖组和吗啡戒断组分别与其他两组相比，差异具有显著性（P〈0．01）；但吗啡依赖组和吗啡戒断组相比，差异无显著性（P〉0．05）。结论：正常大鼠触液核内表达TRPC6；触液核可能通过上调TRPC6的表达参与吗啡依赖和戒断的形成。

英文摘要：

Objective： To investigate the role of cerebrospinal fluid contacting nucleus （CSF-CN） of rat and the changes of TRPC6 expression in morphine dependence and withdrawal. Methods： Male Sprague-Dawley rats were randomly divid- ed into four groups： the normal group; the saline group; the morphine dependence group and the morphine withdrawal group. All animals in each group were tested the morphine withdrawal-like behavioral signs by total withdrawal scores （TWS）. The expression of TRPC6 in CSF-CN in rats was observed by CB-HRP injection into lateral ventricle combined the double-labeled immunofluorescent technique （CB-HRPP/TRPC6）. Results ： TWS of the four groups were 2.0 ± 0.6, 2.1 ± 0.7, 2.6 ± 0.7 and 41.0 ± 4.6, the difference was significant in the morphine withdrawal group compared with other groups（ P 〈0.01 ）. A large number of CB-HRP labeled neurons were found in all groups after CB-HRP injectioninto lateral ventricle. Some neurons of CSF-CN was observed to be TRPC-6-positive. The numbers of CB-HRP/TRPC6 double labeled neurons in the four groups were 81.78 ±.93, 79.44 ±09,254.61 ±15.36,260.00 ± 12.04, respec- tively. The difference was significant when the morphine dependence group and morphine withdrawal group compared with the normal group and saline group （ P 〈 0.01 ）. But there was no significant between the morphine dependence group and the morphine withdrawal group （ P 〉 0.05 ）. Conclusion： TRPC6 was expressed in CSF-CN of the normal rats. CSF-CN might participate in the development of morphine dependence and withdrawal by the up-regulated expression of TRPC6.