植物激素 abscisic 酸(骆驼毛的织物) 在植物生长,开发,和改编起一个关键作用到各种各样的压力条件。细胞的骆驼毛的织物水平常常被适应对改变生理、环境的条件作出回应。迄今为止,为调整骆驼毛的织物层次的机制留下逃犯。这里,我们报导那 BGLU10, -glucosidases, 的一个 multigene 家庭的一个成员在 Arabidopsis 贡献干旱忍耐。T-DNA 插入异种 bglu10 展出了干旱敏感的显型,由水损失,和更低的叶的增加的率描绘了骆驼毛的织物的温度, -glucosidase 活动,骆驼毛的织物内容,和表情 -- 并且在干旱应力下面的干旱应答的基因。相反,线 overexpressing BGLU10 比的显示出更大的干旱抵抗野类型经由蒸发由减少的水损失出现在干旱下面的骆驼毛的织物应答、压力应答的基因的更高的 -glucosidase 活动,骆驼毛的织物水平,和表情强调。在叶肉房间原物的 BGLU10::GFP 和 -TIP1::RFP 的短暂表示证明 BGLU10 酶蛋白质对液泡局部性。同时, BGLU10 在各种各样的机关被表示,并且被几个不能生活的压力导致,建议 BGLU10 可以涉及许多压力回答,并且 ABA-GE 的那个水解作用生产在植物压力反应的免费骆驼毛的织物。
The phytohormone abscisic acid (ABA) plays a critical role in plant growth, development, and adaptation to various stress conditions. The cellular ABA level is constantly adjusted to respond to changing physiological and environmental conditions. To date, the mechanisms for fine-tuning ABA levels remain elusive. Here, we report that BGLU10, a member of a multigene family of β-glucosidases, contributes to drought tolerance in Arabidopsis. The T-DNA insertion mutant bglulO exhibited a drought- sensitive phenotype, characterized by an increased rate of water loss, and lower leaf temperature, β-glucosidase activity, ABA content, and expressions of ABA-and drought-responsive genes under drought stress. In contrast, lines overexpressing BGLUIO showed greater drought resistance than that of the wild-type, as shown by decreased water loss via transpiration, higher D-glucosidase activity, ABA level, and expressions of ABA- and stress-responsive genes under drought stress. Transient expression of B GLU10::GFP and γ-TIP I::RFP in me sophyll cell protoplasts showed that the B GLU 10 enzyme protein was localized to the vacuole. Meanwhile, BGLUIO was expressed in various organs, and was induced by several abiotic stresses, suggesting that BGLU10 may be involved in a variety of stress responses, and that hydrolysis of ABA-GE produces free ABA in the plant stress response