中文摘要：

用＂点击化学＂方法,将炔基化3-氨基苯硼酸与叠氮化硅胶反应,制备了新型硼酸固相萃取吸附剂。采用固相萃取（SPE）对样品进行前处理,反相高效液相色谱分离,紫外检测,建立了一种快速、高效、灵敏的多巴胺分析方法。固相萃取的最优条件为：对200 mg吸附剂装填的萃取柱,用甲醇活化,磷酸盐缓冲溶液（pH 8.0）平衡,再分别用1 mL水和2 mL 20%甲醇淋洗,3 mL 1 mol/L醋酸甲醇溶液洗脱。采用C18反相色谱柱,50 mmol/L磷酸二氢钠-乙腈-甲醇流动相（7∶2∶1,V/V）和检测波长280 nm的色谱条件,对洗脱液进行色谱分析。结果表明,该吸附剂对顺式邻羟基化合物有良好的识别能力。在最优萃取条件下,多巴胺的回收率达90%以上。多巴胺分析的线性范围为0.1～100 mg/L,检出限为0.0001 mg/L,相对标准偏差（RSD）〈10.6%。在实际样品分析中,大鼠空白血加标液的回收率均高于97%,相对标准偏差为4.30%。

英文摘要：

A new boronic acid-functionalized solid phase extraction （SPE） sorbent was prepared by the reaction of 3-aminophenyl boronic acid with azide-functionalized silica through ＂ click chemistry＂. A SPE- HPLC-UV method was developed for the determination of dopamine. The solid-phase extraction conditions were optimized as following： the extraction column packed with 200 mg of sorbent was activated by methanol and equilibrated with phosphate buffer （ pH 8.0 ） , and then the loaded sample was washed with 1.0 mL of water followed by 2.0 mL of 20% methanol aqueous solution to remove interferences and finally the dopamine was eluted with 3 mL of 1.0 mol/L acetic acid in methanol solution. Using Cls reversed phase chromatographic column, dihydrogen phosphate buffer （50 mmol/L ; sodium salt） -acetonitrile-methanol （7 ： 2 ： 1, V/V） as the mobile phase, the eluent was analyzed by HPLC at wavelength of 280 nm. The results showed that the sorbent had excellent recognition ability toward cis-diols compounds. Under the optimized extraction conditions, the recovery of dopamine was up to 90%. Calibration curves were linear in the 0. 1 - 100 mg/L concentration range, the detection limit was 0. 0001 mg/L and relative standard deviation （RSD） was 〈 10.6%. The recoveries of spiked target compound in rats blood blank were higher than 97%, and relative standard deviation （ RSD, rt = 5） was 4.30%.