中文摘要：

探讨甲醛对植入后小鼠胚胎发育毒性及时间一效应关系。方法：应用体外全胚胎培养模型，观察终浓度为81／~mol·L11的甲醛与昆明种小鼠全胚胎共培养不同时间（分别为0，16，26，48h）对胚胎生长发育的影响。结果：甲醛对小鼠胚胎的器官形成早期具有明显的影响，呈现明显的时间·效应关系。甲醛暴露16h则能引起胚胎发育迟缓。当甲醛暴露时间为26和48h时，胚胎生长发育和形态分化等各项指标与对照组（0h）比较均呈现出显著差异（P〈0．05，P〈0．01）；胚胎畸形发生率也显著升高，主要表现为神经管闭合不全、体位翻转不全、脑、心脏、腮弓发育异常及视觉系统、前肢芽、后肢芽、体节等畸形。结论：甲醛对小鼠胚胎生长发育和器官形态分化均有明显的抑制作用，表现出明显的时间·效应关系。

英文摘要：

Objective： To study the time-effect relationship of mice embryonic toxicity and terato- genicity induced by HCHO in vitro, nethods：Kunming mice embryos （8.5 days old） were incubated to- gether with formaldehyde （81μmol.L^-1）for 0, 16, 26 or 48 hours, respectively. The development of mice embryos was then assessed. Results：Formaldehyde degenerated early embryonic growth in a time- dependent manner. The embryonic development was significantly retarded post a 16-hours exposure of formaldehyde. The embryonic development and histomorphological differentiation were significantly sup- pressed post 26-and 48-hours exposure of formaldehyde, manifesting higher incidence rate of teratogenici- ty and embryonic dysmorphogenesis including neural tube defects, brain defects, abnormal heart, abnor- mal arch and small limb bud. Conclusion： Formaldehyde had a significantly conspicuous inhibitory ac- tion of embryonic development and morphological differentiation in a time-dependent manner.