中文摘要：

目的：观察丹酚酸B（SalB）预处理的内皮祖细胞（EPCs）不同比例与骨髓间充质干细胞（BMSCs）联合心脏移植后BMSCs的存活状况。方法：通过结扎左冠状动脉复制大鼠急性心肌梗死模型;密度梯度离心法和贴壁筛选法培养、纯化EPCs与BMSCs,免疫细胞化学法鉴定细胞;丹酚酸B最佳药物浓度处理的EPCs以不同比例与5-溴脱氧尿核苷（Brdu）标记的BMSCs混合,移植至心肌梗死区。4周后,硝基四氮唑蓝（N-BT）染色法测算心梗面积;免疫组化法检测Brdu的表达情况。结果：8：1组和模型组心肌梗死面积分别为（9.23±2.35）%和（19.60±3.23）%,8：1组与其他各组比较心梗面积显著缩小（P〈0.05）。4：1组和8：1组BMSCs阳性细胞数与其他治疗组比较,存活细胞显著增多（P〈0.05）。结论：丹酚酸B预处理的EPCs联合BMSCs的移植,能够提高BMSCs在梗死周边和缺血局部的存活。随着EPCs移植比例的增加,BMSCs的存活数量增多。

英文摘要：

Objective： To investigate the survival of bone marrow mesenchymal stem cells transplantation in acute myocardial infarction rats,the endothelial progenitor cells preconditioned by salvianolic acid B blend with the bone marrow mesenchymal stem cells on different proportion.Methods： The rat model of AMI was produced by ligation of left anterior descending artery.EPCs and BMSCs in vitro were cultivated and purified by density-gradient centrifugation and plastic adherence methods,and identified by immunocytochemistry.BMSCs marked in Brdu and EPCs preconditioned by the optimum salvianolic acid B were injected into the area of the infarct.After 4 weeks,we examined the infarcted heart area by the N-BT Staining Method and the expression of Brdu by immunocytochemistry.Results： 1.The myocardial infarct area were respectively（9.23±2.35）% and（19.60±3.23）% in 8：1 group and model group.8：1 group was decreased remarkably compared with all groups.2.The positive BMSCs count in 4：1 group and 8：1 group were increased remarkably compared with other treatment groups.Conclusions： EPCs preconditioned with Salvianolic acid B can increase BMSCs Surviving by EPCs combined with BMSCs transplantation.With increasd the proportion of EPCs,the Surviving BMSCs quantity were markedly larger in vivo.