中文摘要：

目的利用HPLC—DAD法建立苦碟子中木犀草素7-O-β-D-葡萄糖苷（LGCOP）、木犀草素7-O-β-D-葡萄糖醛酸苷（LGCRP）和芹菜素7-O-β-D-葡萄糖醛酸苷（AGCRP）的测定方法。方法Diamonsil C18色谱柱（200mm×4．6mm，5μm）；流动相为（0．05％甲酸-水）-（0．05％甲酸-乙腈）；二元梯度洗脱：检测波长340nm；体积流量1．0mL／min；柱温35℃；进样量10μL。结果在选定色谱条件下线性关系良好（r≥0．9999）。平均回收率分别为100．5％、100．1％、100．8％，RSD值分别为O．4％、0．3％、0．7％。结论该分析方法能简便、快速地测定苦碟子中黄酮类成分，可为评价不同产地、药用部位及采收期的药材提供科学依据。

英文摘要：

Objective To establish an analysis method for simultaneous determination of luteolin7-O-β-D-glucopyranoside （LGCOP）, luteolin7-O-β-D-glucuronopyranoside （LGCRP） and apigenin7-O-β-D-glucuronopyranoside （AGCRP） in lxeris sonchifolia by HPLC-DAD. Methods HPLC was carried out on a Diamonsil Ci8 （ 200 mm ×4.6mm, 5 μm） column with （0.05% formic acid- water） - （0.05% formic acid - acetonitrile） as mobile phase for two elements of gradient elution. The detection wavelength was set at 340 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 35 ℃. Results There were good linear relationship ofLGCOP, LGCRP, and AGCRP （r≥0.999 9）. The average recoveries were 100.5%, 100.1%, and 100.8% with RSD values of 0.4%, 0.3%, and 0.7%, respectively. Conclusion The method is convenient, specific, and can be used for the quality control of flavonoids in L sonchifolia from different areas, various medicinal parts, and different harvest.