采用常规分离培养和琼脂块拮抗性测定法,研究了西北旱作农业区不同覆盖模式和根系对小麦田土壤中拮抗性放线菌分布的影响.结果表明:①小麦田土壤拈抗性放线菌比率随覆盖模式而异.在SDSA培养基上以覆盖模式较高,常规对照最低,拈抗性放线菌比率由高到底顺序为垄沟(49.1%)〉覆膜(41.5%)〉覆草(37.5%)〉常规(25.0%),垄沟、覆膜和覆草分别较对照提高96.4%、66.O%和50.0%.②小麦根系生长和分泌物对拈抗性放线菌比率有显著影响.在3种覆盖和常规对照条件下,拮抗性放线菌总数的比率、抗细菌和抗真菌的拮抗放线菌比率均表现为根区高于根外,其中覆膜模式下抗真菌放线菌的比率在根区土壤中约为根外的4倍.图1表2参19
Distribution of antagonistic actinomycetes under different mulching conditions and wheat roots in dry farming region of northwestern China was studied by using the normal isolating and agar block methods. The results indicated: (1) Antagonistic actinomycetes differed in percentages with different mulching modes in wheat field. On the SDSA media, their percentage was higher under mulching and that of CK was lowest. The percentage order of antagonistic actinomycetes was RFC (49.1%)〉PFM (41.5%)〉SM (37.5%)〉CK (25.0%). The proportions of antagonistic aetinomycetes under ridge-furrow cultivation (RFC), plastic film mulching (PFM) and straw mulching (SM) were 96.4%, 66.0% and 50.0% higher than CK, respectively. (2) The percentage of antagonistic actinomycetes was remarkably affected by the growth and secretion of wheat roots. With the three mulching modes and CK, the percentages of total antagonistic actinomycetes, antibacteria and antifungi within wheat rhizosphere soil were higher than those outside. The proportion of antifungal actinomycetes in wheat rhizosphere was 4 times higher than that outside under the plastic film mulching. Fig 1, Tab 2, Ref 19