采用荧光紫杉醇直接荧光标记,研究了腹毛类纤毛虫阔口游仆虫(Euplotes eurystomus)中由纤毛器、纤毛器附属微管和非纤毛器微管组成的皮层微管胞器.其中纤毛器、纤毛器附属微管包括口围带及小膜托架微管,波动膜及基部微管骨架网,额腹横棘毛、左缘棘毛及基部的前纵维管束、后纵微管束和横微管束,背触毛及基部的玫瑰花环附属微管等;非纤毛器皮层微管包括背腹面的细小网格状微管,背面的斜向微管层及贯穿于细胞纵长的纵微管层等.形态发生中,老口围带被前仔虫继承,前、后仔虫的额腹横棘毛发生及分化过程中老纤毛器基部微管退化,并且老棘毛及其附属微管可能具有定位和定向作用,但未见直接的物质联系.所得结果为揭示腹毛目纤毛虫皮层微管胞器建构的多样性、细胞皮层微管的分化及其细胞调控提供了基础资料.
The cortical microtubular organelles of hypotrichous ciliate Euplotes eurystomus were visualized by using FLUTAX direct-fluorescent labeling.These organelles were composed of ciliature microtubules,ciliature base-associated microtubules and cortical non-ciliated regions microtubules. The ciliature microtubule and ciliateure base-associated microtubule consists of adoral zone of membranelles(AZM) and membranelle brackets microtubules;undulating membrane (UM) and its base-associated microtubule nets;frontal-ventral-transverse-caudal cirri (FVTCC),left marginal cirri(LMC) and the anterior longitudinal microtubules(ALM),posterior longitudinal microtubules(PLM),transverse microtubules(TM);the dorsal kineties(DK) and the rosette-like skeletal structure.The cortical non-ciliated regions microtubule comprises leptos microtubule nets in the dorsal and ventral cortex;the oblique microtubules in the dorsal cortex;and the longitudinal microtubules.During the process of the morphogenesis of the ciliatures, the AZM of proter is generated from the old AZM.The old ciliature base-associated microtubules degenerate as the FVTCC of proter and opisthe morphogenesis and differentiate.The old cirri and its associated microtubules might play a role of orientation and localization,but without direct physical contribution.The results provide data for the understanding of the construction of cortical microtubular organelles,the differentiation of cortical microtubules and the regulation of cell in ciliates.