中文摘要：

目的了解骨关节炎患者关节软骨内caveolin-1的表达情况，以及软骨降解因子白细胞介素（IL）-1β是否促进软骨细胞表达caveolin-1。方法对8例骨关节炎患者膝关节软骨内caveolin-1基因表达的定量分析采用实时逆转录聚合酶链反应（RT-PCR），caveolin-1蛋白的表达采用免疫组化分析。选用12周龄的雄性SD大鼠加只，通过切断膝前交叉韧带和内侧副韧带制备骨关节炎模型，术后动态观察关节软骨的病理学变化和caveolin-1的表达情况。离体培养的骨关节炎软骨细胞内caveolin-1基因表达量分别采用RT-PCR和实时RT-PCR分析，caveolin-1蛋白表达量采用Western印迹分析。结果与关节软骨轻微损害部位相比，8例骨关节炎患者中有6例患者的关节软骨严重损害部位内caveolin-1 mRNA表达增高约2倍，另外2例无明显差异。免疫组化分析显示，上述6例患者关节软骨严重损害部位内caveolin-1蛋白的表达也增高。随着术后时间延长，大鼠骨关节炎关节的软骨逐渐出现损害，且caveolin-1的表达也持续增高。IL-1β（10ng／ml）可以上调培养的骨关节炎软骨细胞内caveolin-1基因和蛋白表达，并可持续至少48h。结论骨关节炎软骨损害程度与caveolin-1的表达水平具有相关性，IL-1β可以刺激软骨细胞表达caveolin-1。提示IL-1β可能通过诱导caveolin-1表达而促进骨关节炎进展。

英文摘要：

Objective To study the expression level of caveolin-1 in articular chondrocytes in patients with osteoarthritis （ OA ）, and whether catabolic factor IL-1β stimulate caveolin-1 expression in articular chondrocytes. Methods In human OA cartilage, caveolin-1 mRNA was investigated by quantitative real-time RT-PCR while caveolin-1 protein was detected by immuohistologic analysis in 8 cases. OA model was prepared by unilateral anterior cruciate ligament and medial collateral ligament transection in 20 rats. In cultured OA chondrocytes, caveolin-1 mRNA expression was studied by RT-PCR and quantitative real-time RT-PCR, and caveolin-1 protein was analyzed by Western blotting. Results In 6 of 80A cases, human OA articular cartilage, higher expression levels of both caveolin-1 mRNA and caveolin-1 protein were found in advanced degenerated cartilage than less degenerated cartilage of the same joints. In rat OA model, upregulated caveolin-1 expression was found, which was associated with the degree of cartilage destruction. IL-1β （10 ng/ml） upregulated caveolin-1 mRNA/protein expression in cultured OA chondrocytes for at least 48 hours. Conclusion Enhanced expression level of caveolin-1 is associated with cartilage degeneration in OA. IL-1β stimulates caveolin-1 expression in articular chondrocytes, which may be responsible for OA development.