优化超声波提取梵净山石斛(Dendrobium fanjingshanense)多糖的最佳工艺条件,可为梵净山石斛多糖的开发应用提供参考。以梵净山石斛为原料,考察料液比(g∶m L)、提取温度、提取时间对梵净山石斛多糖提取率的影响,并采用正交试验对梵净山石斛多糖提取工艺条件进行优化。结果表明,各因素对梵净山石斛多糖提取率的影响顺序为料液比〉提取时间〉提取温度。最佳提取工艺为料液比为1∶35,提取温度为60℃,提取时间为40 min。在最佳工艺条件下,超声波对梵净山石斛多糖的提取率为9.83%。
The ultrasonic-assisted extraction of polysaccharides from Dendrobium fanjingshanense was optimized with orthogohal test. The effects of the ratio of material to solution, extraction temperature, extraction time on extracting polysaccharides were studied. The factors affecting the extraction of polysaccharides from Dendrobium fanjingshanense were in the order of the ratio of material to solution 〉 extraction time〉extraction temperature. The optimal conditions were the ratio of material to solution of 1:35(mg:L), 60 ℃ and 40 min. Under these conditions,the rate of polysaccharides extracted was 9.83%.