在中科院长白山生态系统定位站,从地表20cm处采集土壤样品,用两种不同形式的氮肥(NO3--N,NH4+-N和NH4NO3)处理土壤样品,用盆栽试验研究了两年生红松苗木受不同浓度N源影响而产生的根际pH变化及其对根际Fe、Mn、Cu和Zn等微量元素的有效性和吸收的影响。结果表明,与对照处理相比,施加铵态氮使根际pH降低,而施加硝态氮则使根际pH增加。根际pH变化的方向与程度取决于N源及施加的浓度。根际pH的变化对根际微量元素的有效性具有显著影响,进而影响到苗木对养分的吸收利用。根际有效Fe、Mn、Cu和Zn的含量与根际pH呈负相关,而苗木叶中Fe、Mn、Cu和Zn含量与根际有效养分含量呈正相关。图5参11。
Dark brown forest soil was collected from the upper 20 cm soil layer in Changbai Mountain Research Station of Ecosystem, Chinese Academy of Sciences. The soil was amended with two different forms of nitrogen fertilizers: NO3- as Ca(NO3)2, NH4+ as NH4Cl at the concentrations of 50, 100, 200 and 400 mgkg-1 respectively. The experiment was carried out with 2-yr-old Pinus koraiensis seedlings in pot. The pH change of rhizosphere soil and the contents of available Fe, Mn, Cu, and Zn in soil and leaves were analyzed. The result indicated that the addition of NH4--N decreased the rhizosphere pH value, while the addition of NO3--N increased the rhizosphere pH value in contrast with the control treatment. The direction and extent of the pH change mainly depended on N source and its concentrations applied. The rhizosphere pH change had a remarkable influence on the availability of the micronutrients in the rhizosphere, and thereafter affected the nutrient uptake by the seedlings. The contents of available mineral nutrients had a negative correlation with the pH value in the rhizosphere soil. The contents of available mineral nutrients in leaves were positively correlated to the levels of the available nutrients in the rhizosphere soils.