中文摘要：

制备具有良好免疫原性的人工抗原是建立测定小分子化合物的免疫分析方法的最关键步骤。本文对半抗原的设计、修饰（修饰位点的选择、修饰物的制备、连接臂的选择）、载体的选择、半抗原与载体的偶联（偶联方式、偶联条件、最佳偶联率）、人工抗原的纯化与鉴定等方面作了详细的介绍。

英文摘要：

The key step to develop an immunoassay for small molecular compound is to synthesize artificial antigens with good immunogenicity. In this article,the hapten design and modification （e.g. the choice of modified sites,preparation of hapten derivative,choice of linking spacer）,selection of carrier,coupling of hapten derivative to carrier （including coupling method,coupling conditions,the best coupling rate ）,purification and identification of artificial antigens were reviewed.