中文摘要：

目的分析贮存红细胞中一氧化氮浓度的改变与临床输注效果的关系,为进一步提高输血的安全性和有效性提供依据。方法 535份红细胞悬液保存期分别为：2d40份,7d120份,14d235份,28d105份,34d35份;分别输注给266位珠蛋白生成障碍性贫血患者,并分析输注的有效率。采用一氧化氮荧光探针技术检测不同保存期贮存红细胞中一氧化氮浓度,并比较临床输注效果。结果随着红细胞制剂贮存期延长,一氧化氮水平有持续下降的趋势（P〈0.01）;红细胞制剂输注有效率也随之下降（χ2=17.41,P〈0.01）,差异有统计学意义。结论随着保存期的延长,贮存红细胞悬液中一氧化氮浓度明显下降,直接影响临床输注有效率。

英文摘要：

Objective To analyze the relationship between the concentrations of nitric oxide（NO） in stored red blood cells and the transfusion effect.Methods The storage life of 535 cases of red blood cells suspension liquid respectively were 2 days of 40,7 days of 120,14 days of 235,28 days of 105 and 34 days of 35,which were respectively transfused to 266 patients,and the effectiveness of blood transfusion was analyzed.The concentrations of NO in different shelf storages of red blood cells were assayed by NO fluorescence probe,and the clinical effect of blood transfusion was compared.Results With the increment of the shelf life of red blood cells,the concentrations of NO continued to decline（P〈0.01） and the efficiency of transfusion also reduced（χ2 =17.41,P〈0.01）.Conclusion With the extension of shelf life,the concentration of nitric oxide in stored red blood cells might decrease significantly,which could directly impact on clinical transfusion efficient.