中文摘要：

目的：研究地卓西平对吗啡依赖大鼠部分脑区突触素ImRNA表达的影响。方法：雄性SD大鼠24只，随机等分为2组。吗啡组大鼠腹腔注射盐酸吗啡，2次／d，起始剂量5mg／kg，逐d递增5mg／kg，至第10d达50mg／kg；干预组大鼠在每次注射吗啡（同吗啡组）前30min腹腔注射地卓西平0．075mg／kg。末次注射后3h及72h，2组各取6只大鼠取脑并冰冻切片，留取中脑腹侧被盖区（VTA）、伏隔核（NAc）、中脑导水管灰质（PAG）、杏仁核（AMG）、海马CA1区（HIPCA1）的脑片，利用原位杂交技术检测突触素ImRNA的表达。结果：末次注射后3h．干预组大鼠AMG、HIPCA1突触素ImRNA的表达高于吗啡组；72h时，干预组大鼠NAc、HIPCA1突触素ImRNA的表达高于吗啡组（P均〈0．05）。其他脑区2组间差异无统计学意义（P〉0．05），结论：地卓西平可促进吗啡依赖大鼠部分脑区突触素I基因的表达，这可能是其抑制吗啡依赖效应的机制之一。

英文摘要：

Aim： To explore the effect of dizocilpine on synapsin I gene expression in defined brain regions of morphine dependent rats, Methods： Twenty-four male SD rats were randomly and equally allocated into morphine group and intervention group. Rats in morphine group were abdominally injected morphine from a dose of by 5 mg/kg and increased by 5 mg/kg per day till increased to 50 mg/kg at the 10th day,twice per day. Rats in intervention group were treated as the morphine group and given dizocilpinc 0. 075 mg/kg at 30 min before morphire injection. At 3 h and 7 h after the last injection, the rat brain tissue was removed,and the synapsin I mRNA levels in ventral tegmental area （VTA） , nucleus accumbens （NAc） , periaqueductal gray （PAG）, amygdala （AMG） , hippocampus CA1 （HIPCA1） were determined by in situ hybridization. Results： The synapsin I mRNA levels of intervention group in AMG and HIPCA1 at the time-point of 3 h, and in NAc and HIPCA1 at the time-point of 72 h were significantly higher than those of morphine group. Conclusion： Dizocilpine may enhance the gene expression of synapsin I in defined brain regions of morphine dependent rats, which might be one of the mechanisms of dizocilpine attenuating morphine dependence.