中文摘要：

采用新型光源高压汞灯制备了三肽分子谷胱甘肽的分子印迹聚合物（MIPs），反应时问缩短，制备条件温和。对MIPs做了一系列的静态吸附实验，结合荧光测定法研究了不同单体一模板比例下MIPs表现出来的吸附性能，对最佳比例制备的MIPs进行了一系列性能研究，包括吸附等温线的测定、Seatehard分析以及薄层色谱分离实验等。结果表明，所合成的MIPs对GSH具有良好的吸附和选择识别能力，最大吸附量为45.4mg／g，特异因子达到了4.98。

英文摘要：

GSH molecularly imprinted polymers were synthesized with UV spot high pressure mercury lamp. This realized the preparation of MIPs in which the template molecules were unstable by means of reducing the time of polymerization from 48 to 2 h at a low temperature. A series of binding experiments in static state was done and the binding performances of MIPs with different molar ralios of templates to monomers was observed by using fluorescence measurement. The character trials which involved the determination of the binding isothermal, the analysis of Scatchard plot and the TLC separation tests were carried out on the MIPs in which the molar ratio of templates to monomers was 1：8. In these experiments, the MIPs showed comparatively excellent characters. For example, the binding amounts reached 45.4 mg/g and the particularity factors 4. 98 were achieved in binding experiments. From Scatchard analysis, it can be known that there were two sorts of binding sites of which the saturated binding amounts were 20. 2 and 64. 5 mg/g and the equilibrium dissocia- tion constants were 0. 435 and 1.48 mmol/L, respectively. The MIPs represented the best separation ability in TLC tests that was attested by the most separation factors of Gly or Glu to GSH on the TLC of MIP, NMIP and silica gel.