欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的测定并分析湖北钉螺（Oncomelania hupensis）线粒体基因组全序列。方法利用特异引物和通用引物分别扩增湖北钉螺线粒体细胞色素C氧化酶Ⅰ（COⅠ）、细胞色素b（Cytb）、16SrRNA（16S）和细胞色素C氧化酶Ⅲ（COⅢ）基因片段,在此基础上利用长PCR技术扩增上述4个基因间的长片段,纯化克隆后采用引物步移法测序。结果湖北钉螺线粒体基因组全序列为15182bp（GenBank登记号为FJ997214）,为闭合环状分子,A＋T含量为67.3%;包括13个蛋白基因、22个tRNA基因、2个RNA基因和一段72bp的A＋T富集区;蛋白质编码基因均以ATG为启动子,除呼吸链NADH脱氢酶的第一亚单位（ND1）基因以潜在的T作为终止密码子外,其余基因均以典型的TAA或TAG为终止子;基因重叠区有2处,分别为4bp和7bp;基因间隔区共21处合计145bp,长度范围为1～30bp;22个tRNA中,除2个tRNASer和tRNAGln、tRNAIle以外均能形成典型的二级结构。结论获得了湖北钉螺的线粒体基因组全序列。
英文摘要：
Objective To sequence and analyze the complete nucleotide sequence of the mitochondrial genome of Oncomelania hupensis. Methods Four long fragments were amplified by long PCR using the primers designed based on mtDNA-COⅠ, Cytb, 16S rRNA and COⅢ gene sequences, and sequenced by conserved primer-walking. Rusults The mitochondrial genome （GenBank accession no. FJ997214） was a circular molecule of 15 182 bp with a total A＋T content of 67.32%, and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 tRNA genes, and an A＋T-rich region of 72 bp. All 13 protein-coding genes of the O. hupensis mtDNA used ATG as start codon. Canonical TAA and TAG termination codons were found in 12 protein-coding genes, and the remaining one （ND1） had an incomplete termination codon （T）. Two short gene overlaps were found with a length of 4 bp and 7 bp, respectively. The length of 21 total intergenic region of mtDNA was 145 bp ranging from 1-30 bp. A total of 22 transferring RNA were found, all of which were typical cloverleaf structure except for two tRNA^Ser, one tRNA^Gln and one tRNA^Ile. Conclusion The complete sequence of O. hupensis mitochondrial genome has been determined.
同期刊论文项目
同项目期刊论文
Harnessing the wealth of Chinese scientific literature: Schistosomiasis research and control in Chin
An integrated approach to identifydistribution of Oncomelania hupensis, the intermediate host of Sch
Generalized negative binomial distribution: A promising statistical distribution for Oncomelania Hup
A longitudinal study of comparison of the Kato–Katz technique and indirect hemagglutination assay (I
Settlement extraction over North China Plain with Landsat and Beijing-1 data using an improved water
Invasive Snails and an Emerging Infectious Disease: Results from the First National Survey on Angios
Landscape pattern analysis and Bayesian modeling for predicting Oncomelania hupensis distribution in
Identifying high risk areas of schistosomiasis and associated risk factors in the Poyang Lake region
Concepts in research capabilities strengthening positive experiences of network approaches by TDR in
Remote sensing and geographic information systems in the spatial temporal dynamics modeling of infec
A growing degree-days based time-series analysis for prediction of Schistosoma japonicum transmissio
Geo-spatial hotspots for hemorrhagic fever with renal syndrome and genetic characterization of Seoul
Design and application of the emergency response mobile phone-based information system for infectiou
Spatial epidemiology in zoonotic parasitic diseases: insights gained at the 1st International Sympos
Landscape elements and Hantaan virus–related hemorrhagic fever with renal syndrome, People’s Republi
Strategy formulation for schistosomiasis japonica control in different environmental settings suppor
Field comparison of immunodiagnostic and parasitological techniques for the detection of schistosomi
Comparison of the Kato-Katz technique, hatching test and indirect hemagglutination assay (IHA) for t
Key differntiating features between scrub typhus and hemorrhagic fever with renal syndrome in northe
Spatio-temporal correlation between human and bovine schistosomiasis in China: insight from three na
Effect of temperature on the development of Schistosoma japonicum within Oncomelania hupensis, and h
Effect of floods on the transmission of schistosomiasis in the Yangtze River valley, People's Republ
Spatial epidemiology in zoonotic parasitic diseases: insights gained at the 1(st) International Symp
The effect of temperature on the development of Angiostrongylus cantonensis (Chen 1935) in Pomacea c
Three Gorges Dam and its impact on the potential transmission of schistosomiasis in regions along th
Bayesian Spatio-Temporal Modeling of Schistosoma japonicum Prevalence Data in the Absence of a Diagn
An integrated approach to identify distribution of Oncomelania hupensis, the intermediate host of Sc
Tribendimidine and Albendazole for Treating Soil-Transmitted Helminths, Strongyloides stercoralis an
Landscape genetics: the correlation of spatial and genetic distances of Oncomelania hupensis, the in
China's new strategy to block Schistosoma japonicum transmission: experiences and impact beyond schi
期刊信息
位置：