采用靛酚蓝比色法、硫代硫酸钠滴定法及高锰酸钾滴定法测定了5、7、9月人参根际土壤脲酶、蔗糖酶及过氧化氢酶3种酶的活性,研究不同栽培年限对人参土壤酶活性的影响。结果表明:3种酶的活性在不同年限人参根际土壤各土层中的分布呈现出不同的分布规律。土壤脲酶除了15~25cm土层R2(2年生人参根际土)土壤中的脲酶活性在5、7、9月呈现下降趋势之外,均在7月份脲酶活性最高。R1(1年生人参根际土)土壤中蔗糖酶的活性在3个月份基本呈上升趋势,而R2、R3(3年生人参根际土)土壤的蔗糖酶和过氧化氢酶不同土层具有不同的动态变化,与人参不同生长发育的时期对养分的吸收不同有关。因此,根据土壤酶活性的变化,在人参的不同发育时期,应该采用不同的栽培管理措施,以满足人参对养分的吸收,为栽培人参的高产提供参考依据。
The urease,invertase and catalase activity of rhizosphere soil from cultivated Panax ginsengfields with different years were measured using the indophenol blue colorimetric method,sodium thiosulfate titration and potassium permanganate titration,respectively,the effect of different cultivated years on rhizosphere soil enzyme activities from cultivated Panax ginseng fields were studied.The results showed that the urease,invertase and catalase activity of rhizosphere soil from cultivated Panax ginsengfields had different distributing characteristics.The urease activity of rhizosphere soil fromP.ginsengfields in different cultivated years was the maximum in the July,except that the urease activity of R2(rhizosphere soil of 2-year-old Panax ginseng)in 15-25cm deceased in three seasons.The invertase activity of R1(rhizosphere soil of 1-year-old Panax ginseng)increased in May,July and September,and the invertase and catalase activity of R2,R3(rhizosphere soil of 3-year-old Panax ginseng)had different dynamic change in different soil layer,which were related to the difference of absorption nutrients in the different period of growth and development of Panax ginseng.The cultivation management measures should be applies in the different period of growth and development of Panax ginseng in order to provide enough nutrients according the change of enzyme activity,this knowledge was essential for high-yield cultivation of Panax ginsengin Jilin province.