采用石蜡切片和生化方法研究了罗氏沼虾(Macrobrachium rosenbergii)受精卵的特性。结果表明:受精卵中央区是一团致密的卵黄物质,卵表面初级卵膜内是一层细胞质。表层细胞质与中央区之间是连接紧密的卵黄颗粒。中央区与外周卵黄颗粒紧密相连,同时也透过卵黄颗粒间的细胞质与表层细胞质相连。卵黄物质的组成以蛋白质(18.67±0.22(μg/卵))和脂类(13.52±0.27(μg/卵))为主,分别占受精卵干重的45.86%和33.21%。在受精卵期较高的淀粉酶活力说明糖类在此期为主要的能源,迅速为受精卵的发育提供能量,蛋白酶类酶活力也较高,分解卵黄蛋白以供能,脂类在受精卵期不是主要的能源。罗氏沼虾受精卵期蛋白SDS-PAGE显示了包含卵黄磷蛋白两个亚基在内的75.6-105KD区域内的蛋白亚基含量丰富,是受精卵期蛋白质的主要成分,也是胚胎发育时期的蛋白营养源,此外,其他分子量的蛋白亚基种类也较多。
Characteristics of fertilized egg in Macrobrachium rosenbergii was studied by using histological section and biochemical methods. The results showed that morphologically, a mass of dense yolk substance existed at the center zone of fertilized egg, and a layer cytoplasm lied in the superficial part of the egg. Between them were yolk granules. The center zone connected with either yolk granules or the superficial cytoplasm. Main components of yolk are protein and lipid (respectively, 18.67±0.22 (μg/egg ) , 13.52 ± 0.27 (μg/egg)). Protease and amylase played a crucial role in fertilized egg, provided energy for embryonic development. Low lipase activity indicated that lipid was not the main energy source in fertilized egg. Fertilized egg protein SDS-PAGE indicated that vitellin was the main constituent of fertilized egg protein. Polypeptides,which between 75.6 KD and 105 KD, were main protein source during the embryonic development of Macrobrachium rosenbergii.