中文摘要：

为研究鼠伤寒沙门菌ATCC13311和其诱导耐药株在转录组水平上的差异,使用环丙沙星为诱导药物,采用梯度浓度诱导法,诱导鼠伤寒沙门菌ATCC13311产生耐药性。构建ATCC13311和其诱导耐药株TW4的转录组测序文库并进行Illumina RNA-seq双向测序及数据分析。诱导获得ATCC13311对环丙沙星MIC值为4mg/L的耐药菌TW4。测序获得了4.46G有效数据,通过de novo拼接,获得了311条unigene,平均长度为15 587bp。拼接所得到的全序列长度为4 847 532bp,经预测4 998条基因中有4 902条得到GO注释。采用COG功能将注释基因划分为25类。初步确定TW4有3 434个基因表达量显著上升,32个基因表达量显著下降,7条代谢途径表达差异显著。利用转录组测序技术对鼠伤寒沙门菌ATCC13311和其诱导耐药株TW4进行研究,揭示了鼠伤寒沙门菌耐药性诱导前后差异表达的基因和显著变化的代谢途径,为深入研究细菌耐药分子机制及与细菌代谢途径之间的联系提供重要依据。

英文摘要：

To gain an insight of the difference of transcriptomes of the Salmonella enterica Typhimurium ATCC13311 and its drug-resistance strain.An in vitroinduction experiment was conducted with increasing concentrations of ciprofloxacin to induce a drug-resistance strain.Two transcriptome sequencing libraries were constructed and sequenced by Illumina RNA sequencing technique,and the global transcriptome information was analyzed subsequently.The MIC of the ciprofloxacin of the drug-resistance strain TW4 is 4mg/L.4.46 Gavailable transcriptome data were acquired,311 unigene were obtained by de novo assembly method and the average length was15 587 bp.The length of the whole genome sequence is 4 847 532 bp,annotation analysis indicated4 902 genes of 4 998 genes had homolog in public protein database,all genes with annotation were classified as 25 clusters of orthologous groups of protein.Compared with ATCC13311,upregulation of 3 434 genes,downregulation of 32 genes were observed and 7metabolic pathways markedly changed in TW4.With the transcriptome sequencing of ATCC13311 and TW4,differentially expressed genes and significant changes of metabolic pathways of ATCC13311 and TW4were revealed.This study provide important basis for further investigation of molecular mechanism of bacterial drug resistance and the contact between bacterial drug resistance and metabolic pathways.