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中文摘要：
为研究纳洛酮对小型猪特异性麻醉剂（XFM）麻醉下大鼠大脑皮质c-fos mRNA表达的影响，探讨纳洛酮催醒XFM麻醉大鼠与大脑皮质c—fos基因的关系。将78只SD纯种大鼠随机分为空白对照组、XFM对照组、XFM＋纳洛酮组、XFM＋生理盐水组．采用实时定量PCR技术检测大脑皮质内clos mRNA表达量。结果表明，腹腔注射XFM后引起大鼠大脑皮质CfosmRNA高效表达，各时期c—fos mRNA表达与空白对照组比较差异极显著（P〈0．01）；纳洛酮注射后引起XFM麻醉大鼠大脑皮质cfospnRNA表达量减少，与XFM对照组比较差异显著（P〈0．01或P〈0．05）。结果提示，大脑皮质c—los基因参与了纳洛酮额颃XFM的麻醇作用，纳洛酮抑制XFM诱导大鼠大脑皮质clos基因表达，可能是纳洛酮催醒XFM麻醉大鼠的重要机理之一。
英文摘要：
To investigate relationship between naloxon woke up anaesthetic rat by XFM and c-fos gene,by study on effect of naloxon on the expression of c-fos mRNA in the rat cerebral cortex,78 SD rats were divided randomly into blank control group,XFM group,XFM add naloxon group, XFM add saline group. The contents of c-fos mRNA was measured by RT-PCR in cerebral cortex. The contems of c-fos mRNA was obviously increased in the XFM anesthesia group as compared with blank control group（P〈0.01 ）. The contents of c-fos mRNA was significantly decreased after naloxon administrated as compared with XFM group（P〈0.05 or P〈0.01）. The results indicated that naloxon palinesthesia action was concerned with c-los gene in cerebral cortex. Naloxon attenu- ates expression of c-fos gene in the rat cerebral cortex may be an important mechanism that nalox- on woke up anaesthetic rat by XFM.
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Antagonistic effect of atipamezole, flumazenil and naloxone against anaesthesia with xylazine, trama
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