中文摘要：

目的：研究人关节软骨细胞在体外模型中,氧分压增高对关节软骨细胞的影响。方法：建立过氧化氢诱导人软骨细胞损伤模型,采用CCK-8试剂检测过氧化氢刺激关节软骨细胞后的细胞活力,并通过RT-PCR和Western Blot测定关节软骨细胞中Ⅱ型胶原（COLⅡ）、粘多糖（ACAN）、金属蛋白酶13（MMP13）和含血小板结合蛋白基序的解聚蛋白样金属蛋白酶5（ADAMTS5）等软骨基质相关基因的表达水平。结果：一定量浓度的过氧化氢体外刺激人关节软骨细胞后,在12 h时,细胞活力升高,在24 h时,细胞活力下降;过氧化氢刺激软骨细胞24 h后检测COLⅡ和ACAN,其表达水平下降（P〈0.05）,而MMP13和ADAMTS5表达水平升高（P〉0.05）。结论：过氧化氢模拟的软骨细胞外活性氧浓度增高会影响软骨细胞基质的合成与降解。

英文摘要：

Objective To explore the influence of elevating the oxygen pressure on articular chondro- cytes in vitro. Method A hydrogen peroxide induced human articular chondrocyte damage model was es- tablished. Then the articular chondrocyte viability was detected using the CCK-8 kit. Collagen Ⅱ （COL Ⅱ ）,The expression levels of aggrecan （ACAN）, matrix metalloproteinase 13 （MMP13） and adis- integrin and metalloproteinase with thrombospondin motifs 5 （ADAMTSS） were detected using the real- time PCR and Western blotting. Result The viability of articular chondrocytes improved at 12 h but de- creased at 24 h after the stimulation of hydrogen peroxide. Twenty-four hours later,the average expres- sion level of COL Ⅱ and ACAN decreased（P〈0.05）,while that of MMP13 and ADAMTS5 elevated（P〉 0.05）. Conclusion Hydrogen peroxide induced elevation of the extracellular oxygen pressure can influ- ence the synthesis and degradation of the articular chondrocyte extracellular matrix.