中文摘要：

【目的】利用不同定位方法，对不同环境条件下大豆异黄酮主要组分进行QTL定位研究，为大豆异黄酮分子标记辅助育种提供理论依据。【方法】以异黄酮含量有显著差异的鲁黑豆2号（3697．24μg·g^-1）和南汇早黑豆（1816．67μg·g^-1）为亲本构建的F5：7-8。重组自交系为材料，分析RIL群体的SSR标记多态性，结合HPLC法鉴定异黄酮主要组分含量。【结果】绘制了一张包含161个多态性SSR标记，全长3546．54cM的大豆遗传连锁图谱。利用ICIMapping 3．2软件的ICIM、IM和SMA3种定位方法，共定位到4种环境下与异黄酮主要组分相关的14个QTL。【结论】3个标记区间在多个环境和多种定位方法下均被检测到，分别是Sat_003-satt306、Satt070-Satt122和Satt571-Satt270。

英文摘要：

[Objective] The QTLs for major isoflavone components were identified among multiple environments by different QTL mapping methods in soybean seeds, in order to provide a theoretical basis for soybean isoflavone marker-assisted selection. [Method] In this study, a recombinant inbreed line population （RIL, F5： 7-8） was developed by using the cross between the isoflavone contrasting cv. LHD2 with high-isoflavone concentration （3 697.24μg·g^-1） and cv. NHZ with low-isoflavone concentration （1 816.67μg·g^-1）. The polymorphism of SSR markers among the RIL population was analyzed and the major isoflavone components were determined by HPLC. [Result] The results showed that a soybean linkage map with a total distance of 3 546.54 cM was constructed using 161 polymorphism SSR molecular markers in the RIL population. A total of 14 QTLs associated with the major isoflavone components were found by ICIM, IM, and SMA methods in the ICIMapping 3.2 soitware under the four environments. [Conclusion] The three QTLs flanked by the marker interval Sat_003-Satt306, Satt070-Satt122 and Satt571-Satt270 were detected among multiole environments in more than two methods.