中文摘要：

将γ-（甲基丙烯酰氧）丙基三甲氧基硅烷（MPS）接枝到硅胶表面，然后以异丙威为模板分子，甲基丙烯酸（MAA）为单体，乙二醇二缩水甘油醚（EGDE）为交联剂，制备了异丙威表面分子印迹材料MIP—PMAA-MPS-SiO2。采用动态与静态两种方法研究了该印迹材料对异丙威的结合性能与分子识别特性。结果表明，MIP—PMAA-MPS-SiO2印迹材料对模板分子异丙威具有良好的特异性识别作用，相对于参比物速灭威和甲萘威，印迹材料对异丙威的选择性系数分别为6．73和8．95。经过Scatchard模型分析，计算得到两类结合位点的最大表观吸附量分别为230和117mg／g，印迹聚合物的平衡离解常数蚝分别为1428．6和476．2mg／L。油菜样品的加标回收实验表明，此印迹材料可以对痕量异丙威进行富集分离测定，且可重复使用。

英文摘要：

The silylating agent 3-methacryloxypropyl trimethoxysilane （MPS） was grafted on the surface of silica gel particles, then the molecular imprinting was carried out by using isoprocarb as template, methylacrylic acid （MAA） as functional monomers and ethylene glycol diglycidyl ether （EGDE） as crosslinker in the aqueous ethanol solutions, and then the surface-molecular imprinted materials of isoprocarb MIP-PMAA_MPS.SiO2 was obtained. The binding properties of MIP-PMAA_MPS_SiO2 for isoprocarb were studied with both static and dynamic methods. The experiment results showed that MIP-PMAA.MPS.SiO2 had specific recognition selectivity and excellent binding selectivity for the template molecule. In respect to the reference substance tsumacide, its selectivity coefficient for isoprocarb was 6.73, the relative selectivity coefficient was 4.43. After the Scatchard analysis, Qmax （the apparent maximum binding amount） values were calculated to be 230 and 117 mg/g, respectively, and Kd（ the dissociation constant） values were calculated to be 1428.6 and 476.2 mg/L, respectively. Besides, spiked recovery in rape samples experiment shows that imprinted material of MIP-PMAA-MPS-SiO2 can enrich and separate for trace amounts of isoprocarb, and it is also easy to be used repeatedly.