中文摘要：

背景与目的：卵巢癌的进展是一种多因素、多环节、多阶段的过程，近来研究表明卵巢癌患者白介素8（IL-8）水平显著高于良性肿瘤患者及健康人群，卵巢癌的生长和转移可能与过度表达的IL-8有关，但尚无报道表明IL-8主要亚型在卵巢癌中的作用。本研究探讨IL-8的3种亚型IL-8（1-77）、IL-8（5-77）和IL-8（9-77）对卵巢癌SKOV3细胞的增殖、生长、迁移和侵袭能力的影响及其作用机理。方法：确认分别获得IL-8的3种亚型稳定转染的SKOV3细胞后，应用MTT比色测定法比较各细胞的生长曲线；采用流式细胞仪比较各细胞的细胞生长周期；采用集落形成实验比较各组细胞的克隆率；运用Transwell小室比较各细胞体外迁移和侵袭能力。结果：IL-8（1-77）-pwpi-SKOV彝日胞和IL-8（5-77）-pwpi-SKOV3在G2期的比率明显高于SKOV3（P=0．017，P=0．020）；IL-8（1-77）-pwpi-SKOV3和IL-8（5-77）-pwpi-SKOV3细胞克隆率明显高于SKOV3（P=0．000，P=0．001），IL-8（9-77）-pwpi-SKOV3细胞克隆率明显低于SKOV3（P=0．013）；细胞体外迁移能力多组间差异无统计学意义（P〉0．05），但细胞体外侵袭能力IL-8（1-77）-pwpi-SKOV3和IL-8（5-77）-pwpi-SKOV3明显强于SKOV3（P=0．000，P=0．002），IL-8（9-77）-pwpi-SKOV3明显弱于SKOV3（P=0．067）。结论：IL-8（1-77）和IL-8（5-77）可能促进卵巢癌SKOV3细胞的生长和增殖，并增强其侵袭能力，而IL-8（9-77）对卵巢癌SKOV3细胞增殖可能有抑制作用。

英文摘要：

Background and purpose： Ovarian cancer progression is a multistep process. Recent research shows that IL-8 level of ovarian cancer patients is significantly higher than that of patients with benign tumors and normal people, the growth and metastasis of ovarian cancer may be associated with over-expression of IL-8, but there is no report about the function of the main subtypes of IL-8 in ovarian cancer. The study was to investigate the influence of IL-8 three subtypes on ovarian cancer SKOV3 cell proliferation, growth, migrating and invasive capability, and the underlying mechanism. Methods： After confirming obtaining SKOV3 cell with three hypotypes IL-8（1-77）, IL-8（5-77）, IL-8（9-77） over-expression respectively, MTT colorimetric method was used to detect the growth cure of different cells; The flow cytometry was used to compare the cell cycle of different cells; The cell proliferation capability of different cells was tested by the cell body colony formation; Transwell closet was used to compare different cell body＇s external migration and invasive capability. Results： IL-8（1-77）-pwpi-SKOV3 and IL-8（5-77）-pwpi-SKOV3 cells in the ratio of G2 phase were significantly higher than that of SKOV3 cells （P=0.017 and 0.020）. The cloning formation rate of IL- 8（1-77）-pwpi-SKOV3 and IL-8 （5-77） -pwpi-SKOV3 cells were significantly higher than that of SKOV3 cells （P=0.000 and 0.001）, IL-8 （9-77）-pwpi-SKOV3 cells was significantly lower than SKOV3 cells （P=0.013）; the migration ability in vitro of different groups of cells had no statistically significant differences （P〉0.05）, but the invasion ability invitro of IL-8（ 1-77 ）-pwpi-SKOV3 and IL-8 （5-77） -pwpi-SKOV3 cells were significantly stronger than that of SKOV3 cells（P=0.000 and 0.002）, IL-8 （9-77） -pwpi-SKOV3 cells was significantly weaker than that of SKOV3 cells（P=0.067）. Conclusion： The subtypes of IL-8 （1-77） and IL-8 （5-77） can promote ovarian cancer SKOV3 cell growth and prolif