中文摘要：

目的研究丹酚酸B对活化血小板诱导的人脑微血管内皮细胞（HBMECs）炎症应答的干预效应。方法体外激活的血小板与HBMECs共培养12h，建立炎症应答模型，实时荧光定量RT-PCR检测HBMECs中细胞间黏附分子-1（ICAM-1）、IL-1β、IL-6以及IL-8、趋化蛋白-1（MCP-1）mRNA表达水平。同时采用10μg／mL丹酚酸B预适应HBMECs24h，检测上述炎症介质表达水平的变化。结果丹酚酸B预适应可不同程度减少活化血小板诱导的HBMECs炎症模型中ICAM-1、IL-1B、IL-6、IL-8、MCP-1mRNA表达水平，与干预前比较，差异均有统计学意义（P〈0．05）。结论丹酚酸B可阻抑活化血小板诱导的脑微血管内皮细胞炎症应答，推测是其治疗脑缺血的机制之一。

英文摘要：

Objective To investigate the interventional effect of salvianolic acid B （SalB） on activated platelet-induced inflammatory response in human brain microvascular endothelial cells （HBMECs）. Methods Activated platelets and HBMECs were co-cultured to establish inflammatory response models. The mRNA expression levels of inflammatory mediators, including intercellular adhesion molecule-I （ICAM-1）, IL-1β, IL-6, 1L-8 and monocyte chemoattractant protein-1 （MCP-1） in HBMECs, were detected by quantitative RT-PCR. Cells of the pretreatment group were pretreated with 10μg/ml SalB for 24 h, and the changes of mRNA expression levels of these inflammatory mediators were analyzed. Results The mRNA expression levels oflCAM-I, IL-1β, IL-6, IL-8 and MCP-1 in activated platelet-induced inflammatory response model of SalB pretreatment group were significantly reduced as compared with those without pretreatment （P〈0.05）. Conclusion SalB can repress the activated platelet-induced inflammatory response in HBMECs, which might be one of the mechanisms of anti-cerebral ischemia effect.