中文摘要：

Appressorium 形成是在建立在米饭强风真菌， Magnaporthe oryzae，和它的主人植物之间的一个成功的相互作用的一个重要事件，米饭。发生在 appressorium 区别的分子的事件的理解将给新策略控制米饭强风。从 appressorium 提取全部的 RNA 的一个快、可靠的方法为在 appressorium 形成和它的机制期间学习基因表示是必要的。我们发现那部副本电影是为 appressorium 形成的一个有效基础，甚至当与高密度 conidia 接种时。当在 1 × 1 与 conidia 接种时分生孢子萌芽和 appressorium 形成的百分比是的 0 ⁶ ml ⁻¹,(97.98 ± 0 .67 )% 并且(97.88 ± 0 .45 )% 分别地。在为全部的 RNA 隔离的 appressorium 收集，和差不多 113.6 μg 总计 RNA 以前，我们使用了 Trizol 在接种以后在 24 h 从成熟 appressoria 被孤立。二基因， MNH6 和 MgATG1 的泛函分析，从 cDNA 孤立减少性的图书馆，表明 RNA 的数量足够好构造 cDNA (互补 DNA ) 图书馆或 cDNA 减少性的图书馆。这个方法可能为在哪个 conidia 在主人感染的早阶段区分进 appressoria 的另外的病原的真菌的 appressorium RNA 隔离也是适用的。

英文摘要：

Appressorium formation is an important event in establishing a successful interaction between the rice blast fungus, Magnaporthe oryzae, and its host plant, rice. An understanding of molecular events occurring in appressorium differentiation will give new strategies to control rice blast. A quick and reliable method to extract total RNA from appressorium is essential for studying gene expression during appressorium formation and its mechanism. We found that duplicate film is an efficient substratum for appressorium formation, even when inoculated with high density conidia. When inoculated with conidia at 1 × 106 ml^-1, the percentages of conidium germination and appressorium formation were （97.98±0.67）% and （97.88±0.45）%, respectively. We applied Trizol before appressorium collection for total RNA isolation, and as much as 113.6 lag total RNA was isolated from the mature appressoria at 24 h after inoculation. Functional analysis of two genes, MNH6 and MgATG1, isolated from the cDNA subtractive library, revealed that the quantity of RNA was good enough to construct a cDNA （complementary DNA） library or a cDNA subtractive library. This method may be also applicable for the appressorium RNA isolation of other pathogenic fungi in which conidia differentiate into appressoria in the early stages of host infection.