中文摘要：

采用氯胺＊T法对白蛋白融合干扰素α2b进行^125I标记，PD-10柱纯化，三氯醋酸沉淀法测定放化纯；体外以WISH／VSV系统比较白蛋白融合干扰素α2b和^125I-白蛋白融合干扰素α2b的抗病毒活性；SD大鼠皮下注射^125I-白蛋白融合干扰素α2b，分别于给药后0．5、2、6、24、48、90、180、300h放血处死，取血和相关脏器，称重并测定放射性，计算每克脏器放射性占注入量的百分率（％ID·g^-1）。结果显示，氯胺-T法制备的^125I-白蛋白融合干扰素α2b标记率为82．72％，放化纯95．53％，放射性比活度为0．26MBq／ug；抗病毒活性比较结果表明标记前后的生物学活性几乎无变化；皮下注射大鼠体内分布研究表明，^125I-白蛋白融合干扰素α2b在血中于6h达高峰，消除缓慢，未见特异性组织或器官的浓聚。

英文摘要：

^125I-albumin-IFN-alpha2b was prepared with the methods of Ch-T and purified with PD-10 column. The radiochemical purity was measured with TCA （trichloroacetic acid） precipitation. The antiviral activities of ^125I-albumin-IFN-alpha2b and albumin-IFN-alpha2b were compared with WISH/VSV system in vitro. SD rats were injected with ^125I-albumin-IFN-alpha2b subcutaneously and sacrificed at 0.5, 2, 6, 24, 48, 90, 180 and 300 h post-injection. Selected organs were dissected, weighed and their radioactivity was measured using γ-counter. The accumulated radioactivity in the tissues was calculated in terms of percentage of injected dose per gram organ （%ID.g^-1）. The labeling yield was 82.72%. The radiochemical purity of 125I-albumin-IFN-alpha2b was 95.53%, and its radioactivity was 0.26 MBq/ug. The antiviral bioactivities of albumin-IFN-alpha2b and ^125I-albumin- IFN-alpha2b did not change. Biodistribution analysis of ^125I-albumin-IFN-alpha2b in rats showed that concentrated ^125I- albumin-IFN-alpha2b in blood reached maximum at 6 h post injection, and eliminated slowly. No specific accumulation was seen in other tissues. ^125I-albumin-IFN-alpha2b could maintain in peripheral blood for a long time and it meant albumin-IFN-alpha2b would be an effective long-term interferon.