中文摘要：

将BnTR1基因转化到了本生烟中,发现与非转基因对照相比,转基因烟草对甘露醇和PEG模拟干旱表现出较强的抗性,尤其是PEG胁迫时,三个转基因株系在生物量的积累和整体长势方面都优于对照.干旱处理20d后复水并培养14d后,对照烟草全部枯死,但是所有转基因烟草都恢复生长直至开花结果.当把烟草叶片圆片浸泡在系列盐水中,盐浓度超过600mM时,对照烟草圆片受损白化,而转基因烟草圆片能保持较大面积的绿色。热激蛋白基因HSP90和HSP70在所有烟草中的表达量都有明显提高,其表达量的增加在对照和转基因株系中并无差异,但是在正常条件下不表达的HSF30和sHSP17.6基因在热激处理后都表达了,而且HSF30的表达量增加了50%,sHSP17.6的表达量增加了30%.

英文摘要：

BnTR1was transformed into Nicotiana benthamiana.Compared with the control,this transgenic plant shows a better resistance to mannitol and PEG,especially in growth status and accumulation of biomass under PEG stress.After 20 days under drought treatment,plants were rewatered and then cultivated for 2weeks,and all transgenic Nicotiana benthamiana were recovered from wilting,and all wild type Nicotiana benthamiana died.These results indicated that Nicotiana benthamiana can be resistant to drought with BnTR1.When leaves were treated with hydrosaline solution,transgenic Nicotiana benthamiana could maintain green even as salt concentration was above 600 mM,while control plants became albino.This result suggested that chloroplast and chlorophyll in transgenic Nicotiana benthamiana can be more stable under salt stress,and transgenic Nicotiana benthamianacan be more resistant to salt stress.After heat shock,the expression of HSP90 and HSP70in transgenic Nicotiana benthamiana and controls were all up-regulated,and there were no significant difference in increment between transgenic plants and controls.The expression of HSF30 and sHSP17.6were also induced which can＇t be detected in normal condition,HSF30 increasing 50% and sHSP17.6increasing 30%.