为研究二色补血草的氮素需求,尤其是营养液中氮素形态(NO3^-和NH4^+)和适宜其生长的营养液浓度,进行了2个室内水培试验。1)氮素形态试验:在总氮素浓度为7.14 mmol/L的情况下,设置硝态氮与铵态氮的5种配比即c(NO3^--N)∶c(NH4^+-N)为100∶07、5∶25、50∶50、25∶75和0∶100;2)营养液浓度和氮素形态试验:设置营养液浓度3组倍数处理(1倍、1/2倍和1/8倍,其中总氮素浓度为7.14、3.57和0.90 mmol/L),每组之下再设3种硝铵配比即c(NO3^--N)∶c(NH4^+-N)为100∶0、50∶50和0∶100,共9个处理。处理时间26 d。结果表明:2种形态氮等比例供给时,二色补血草的总干重、地上部干重、总根重、根长密度均显著高于纯硝态氮和纯铵态氮处理;供应纯铵态氮时,生长最差,叶片脯氨酸(植物逆境响应指标)含量显著增加(纯铵态氮比纯硝态氮在1倍、1/2倍和1/8倍营养液浓度下分别增加62.4%、46.6%和111.4%),并出现严重的铵毒症状(幼叶叶缘焦边,根系黑褐坏死);1/2倍标准浓度(其中总氮浓度为3.57 mmol/L)营养液且等比例混合态氮条件下,二色补血草生长最好。根据二色补血草野生土壤条件推测,二色补血草是喜硝态氮植物,本研究证实了这一推论,但最适合其生长的硝铵配比为50∶50。
To determine the nitrogen demand, especially the ratio of nitrogen forms (NH4 ^+ vs NO3^-) and concentration in nutrient solution for the cultivation of Limonium bicolor L., two experiments were carried out under controlled hydroponic culture. The first experiment on nitrogen forms involved treatments with a total nitrogen concentration of 7.14 mmol/L and five NO3^ - -N : NH4^+ -N ratios of 100:0, 75 : 25, 50:50, 25 : 75, and 0 : 100. The second experiment on solution concentration and nitrogen forms had three solution concentrations (1, 1/2, and 1/8 of solution concentration in the first experiment) and total nitrogen concentrations of 7.14, 3.57, 0.90mmol/L, respectively ) combined with three ratios of NO3 ^- -N: NH4^ + -N ( 100: 0, 50: 50, 0: 100). Both experiments lasted 26 days after plants were transplanted into treatment solutions. Results showed that total dry weight, shoot dry weight, root dry weight, and root length density of Limonium bicolor grown under mixed nitrogen combined with NO3^ - -N : NH4 ^+ -N ratio of 50 : 50 were significantly higher than that grown under sole nitrate or ammonium. Plants performed worst and showed serious NH4^+ toxicity (the leaf edge withered and the root turned into black and dead) under sole ammonium solution. The leaf proline content (a signal substance under a stress environment) increased dramatically. Limonium bicolor grew best under mixed nitrogen with a N-ratio of 50:50, and the optimum solution concentration was half of the standard solution concentration with a total nitrogen concentration of 3.57 mmol/L. Limonium bicolor is a plant species preferring nitrate nitrogen under its natural environment. This deduction was confirmed in this research, however, the optimum nitrogen form for its growth was mixed nitrogen with a N-ratio of 50:50.