采用RT-PCR方法检测对水杨酸依赖的信号途径中PR-1、GLU和CHI3个防御基因在转OXO基因与非转基因的油菜中表达差异的结果表明,在转基因油菜株系中这3个基因都有不同程度的上调表达,暗示转OXO基因的油菜抗菌核病的能力增强可能与对水杨酸依赖的信号途径受诱导有关。
Expressions of three defense genes (PR-1, GLU and CHI) of salicylic acid (SA)-dependent signaling pathway were measured by RT-PCR in transgenic oilseed rape (Brassica napus) lines and wild plants. The results showed that the three genes were all up-regulated expressed in transgenic lines, which indicated that the mechanism of enhanced resistance to Sclerotinia sclerotiorum of transgenic lines was related to the induction of SA-dependent signaling pathway.