中文摘要：

以14个花生品种为材料，对花生组织培养及植株再生进行了研究。将花生成熟胚幼叶、子叶和下胚轴外植体接种到添加0—2mg／LNAA和0～10mg／LBAP的MSB，（MS无机盐＋B5有机）培养基上诱导愈伤，再转到添加0～10mg／LBAP的分化培养基上诱导不定芽。结果表明，诱导培养基中添加的激素及其浓度对以后在分化培养基上不定芽分化有重要作用，以添加1mg／LNAA和6mg／LBAP最利于不定芽分化；分化培养基中添加4mg／LBAP利于不定芽伸长及植株再生；幼叶外植体分化不定芽频率明显高于子叶和下胚轴；不同基因型不定芽分化率存在明显差异，白沙1016和花育23幼叶外植体获得了较高的不定芽分化率，分别达到91．8％和88．5％。再生苗经生根培养和驯化后，移栽花盆可正常开花结果。

英文摘要：

Efficient adventitious shoot formation and plant regeneration by tissue cultures in peanut （Arachis Hypogaea L. ） was studied using 14 cuhivars. The immature leaf, cotyledon and hypocotyl explants were cultured on MSB5 induction medium （MS salts and B5 vitamins） supplemented with 0-2 mg/L NAA and 0 - 10 mg/L BAP. Then the formed calli were transferred onto differentiation medium containing 0 - 10 mg/L BAP for shoot induction. The result showed：①the concentration of NAA and BAP added to medium successfully influenced the shoot regeneration frequencies, the suitable induction medium was MSB5 medium added with 1 mg/L NAA and 6 mg/L BAP, and the suitable differentiation medium was MSB5 medium supplemented with 4 mg/L BAP;②the immature leaf had higher frequency of shoot formation than cotyledon and hypocotyl ; ③.the frequencies of shoot formation were different due to the difference in cultivars, the higher frequencies were obtained in cultivar Baisha 1016 （91.8%） and Huayu 23 （88.5%）. The regenerated plants flowered and set pod normally after transplanted in pots.