中文摘要：

目的研究CD133在人舌鳞癌Tca8113细胞系中的表达,观察纯化的CD133肿瘤细胞体外生长特性。方法采用有限稀释法观察单细胞体外增殖的能力;利用超低黏附板,观察舌鳞癌Tca8113细胞体外悬浮肿瘤细胞成球;流式细胞仪检测舌鳞癌Tca8113细胞系中的CD133表达;免疫磁珠分选技术纯化CD133肿瘤细胞,体外培养并观察其增殖及分化能力。结果单细胞体外增殖12 d后,仅有5.23%的细胞具有持续增殖的能力。体外观察发现,超低黏附96孔培养板中细胞悬浮生长,部分可以形成肿瘤球;Tca8113细胞系中有占肿瘤部分0.95%的CD133肿瘤细胞呈阳性表达,且分选出的CD133＋肿瘤细胞的增殖能力均高于CD133-肿瘤细胞及未分选的肿瘤细胞;CD133在培养体系中的比例逐日下降,由培养第1天的92.45%下降至第12天的1.62%。结论 Tca8113细胞系中肿瘤细胞具有异质性,且CD133＋细胞占肿瘤细胞比例较低,在体外分化和增殖能力较强,CD133可能是肿瘤起始细胞的表型标志之一。

英文摘要：

Objective To detect the expression of CD133 in human tongue squamous cell carcinoma Tca8113 cell line and observe proliferation ability of CD133 groups in vitro.Methods Limiting dilution assay was employed to observe the proliferating character of Tca8113 single cell in vitro.The ability of growing as cancer spheroids was observed with ultra-low attachment plates.The flow cytometry was used to detect the expression of putative tumorinitiating cell marker CD133 in human tongue squamous cell carcinoma Tca8113 cell line.The selective technique of immunomagnetic beads was applied to purify CD133 tumor cells,CD133 tumor cells were cultured and their ability of proliferation were observed in vitro.Results After 12 days,the result of single cell culture in vitro revealed that about 5.23% of cultured Tca8113 cells possessed the capacity of continue proliferation.The cells line fromed floating clusters with one week of passaging cells into non-adherent plates.Approximately 0.95% of cells in Tca8113 cell line expressed CD133.Compared with CD133-cells and control Tca8113 cells,CD133＋ cells demonstrated increased proliferation capacity.The proportion of CD133 cells decreased in culture as days passed.The percentage of CD133 ＋ cells decreased from 92.45% to 1.62% in twelve days’culture.Conclusion Tumor stem cells have the character of heterogenity and lower proportion of CD133 but higher ability of proliferation,and the diferentiation in human tongue squamous cell carcinoma Tca8113 cell line in vitro,CD133 may be one of makers for tumor-initiating cell of human tongue squamous cell carcinoma Tca8113 cell line.