中文摘要：

Pteris vittata 罐头亢奋积累砷(作为) 到它没有出现的干燥重量的 】1% ，任何东西毒性签名，显示有效植物内部的 detoxification 机制的存在。尽管 vacuolar 区分被知道在重金属 detoxification 和忍耐起一个重要作用，在这个亢奋的蓄电池的砷区分的直接证据正在缺乏。这里，我们报导潜水艇细胞的本地化作为在 P 的胼胝。vittata。从 P 的配偶体导致的胼胝。暴露于 0.2 mmol/L 砷酸盐 20 天的 vittata 被直接孤立房间墙，原物和液泡检验，并且决定砷集中。是的总数在胼胝，大约 94% 在原物，并且那， 91% 在液泡是在场的，显示液泡是一个主要存储地点为是在 P。vittata。另外，在液泡的化学部件的变化被 Fourier 变换分析红外线的光谱学(FTIR ) 。

英文摘要：

Pteris vittata can hyperaccumulate arsenic （As） to 〉1% of its dry weight without showing any signs of toxicity, indicating the existence of effective plant-internal detoxificaUon mechanisms. Although vacuolar compartmentalization is known to play an important role in heavy metal detoxification and tolerance, direct evidence of arsenic compartmentalization in this hyperaccumulator was lacking. Here we report the subcellular localization of As in the callus of P. vittata. The callus induced from gametophytes of P. vittata exposed to 0.2 mmol/L arsenate for 20 days were examined by directly isolating cell walls, protoplasts and vacuoles, and determining arsenic concentrations. Of the total As in the callus, about 94% was in the protoplast, and of that, 91% was present in the vacuoles, indicating that vacuoles are a major storage site for As in P. vittata. In addition, the changes in the chemical components of vacuoles were analyzed by Fourier transform infrared spectroscopy （FTIR）.