中文摘要：

目的 建立一种新的从人血凝块中提取基因组DNA的方法 。方法 血凝块于室温自然解冻,匀浆,碱消化,酚氯仿抽提,电泳检测。结果 用碱性裂解法从人血凝块中成功提取到基因组DNA,提取的DNA质量浓度平均为0.46 g/L,且吸光度比值A260/A280〉1.8,选取人Gpx-1基因作PCR获得满意的目的 片段。结论 用碱性裂解法提取的基因组DNA的总量较高,提取效率高,PCR扩增效果好,可很好的应用于血凝块基因组DNA的提取。

英文摘要：

Objective To explore a new method to isolate genomic DNA from human blood clot. Methods Blood clot was thawed naturally in room temperature and homogenized, then digested with alkaline and extracted by phenol and chloroform, electrophoresis detection. Results The genomic DNA was successfully extracted by alkaline lysis method. The average quantity of the extracted DNA was 0.46 g/L, while A260/A280〉1.8. PCR was performed using the human Gpx-1 gene, and we obtained the needed target gene fragments. Conclusion The alkaline lysis extraction method is reliable for obtaining high quantities of DNA from blood clot suited for PCR amplification.