中文摘要：

对大亚湾海域表层水体的可培养细菌进行分离,测定了分离培养细菌的16S rRNA基因V3区序列,并采用该研究中的35个序列与从GenBank下载的46个序列构建了系统树,对细菌进行了分子鉴定。在9个站点的表层水体中共分离得到70株菌株,分属35个种。其中α-变形菌纲（Alphaproteobacteria）和放线菌纲（Actinobacteria）为分离菌株最多的两个菌纲,分离率分别为40%和35.7%;而β-变形菌纲（Betaproteobacteria）仅分离到1株（1.4%）。此外,还分离得到盖球菌属（Kytococcus）、间胞囊菌属（Intrasporangium）等非常见菌株。大亚湾海域细菌的Shannon-Wieners多样性指数为3.21。大亚湾海域表层水体可培养细菌丰富的种类多样性以及较高的α-变形菌分离率,说明了该海域具有良好的水质,同时也是潜在的微生物菌种资源库。

英文摘要：

Culturable bacter/a were isolated from surface seawater in Daya Bay, and identified by means of 16S rRNA gene V3 region sequences. A phylogenetic tree was constructed using 35 sequences in this study and 46 sequences from GenBank. Seventy bacterial isolates were isolated and cultured from surface water of 9 stations in Daya Bay, which were classified into 35 categories. Within them, 40% belonged to Alphaproteobacteria, 35.7% to Actinobacteria, and 1.4% to Betaproteobacteria. Meanwhile, two genera, Kytococcus and lntrasporangium, which were rarely reported, were also isolated. The value of Shaunon-Wiener＇s index was 3.21, indicating a diversity of bacteria in Daya Bay. The high diversity of the culturable bacteria and abundance of Alphaproteobacteria suggested the good water quality and the potential microbial resources in Daya Bay.